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PureCube Ni-NTA Agarose 蛋白純化瓊脂糖
PureCube His Affinity Agarose (NTA)是針對(duì)組氨酸標(biāo)簽的親和層析產(chǎn)品，組氨酸標(biāo)簽是應(yīng)用廣泛的親和層析標(biāo)簽，這種標(biāo)簽具有小尺寸、低免疫原性的特點(diǎn)和在多種天然或變性條件下的廣泛適用性，NTA相對(duì)于IDA具有更高的洗脫液純度。
產(chǎn)品優(yōu)勢(shì)：
1）德國(guó)制造，品質(zhì)與信譽(yù)的堅(jiān)實(shí)保證；
2）結(jié)合載量可高達(dá)80mg/mL，目前市面上高結(jié)合能力；
3）更*的DTT和EDTA穩(wěn)定性和耐受性；
4）凝膠的多孔性和均勻粒徑，使蛋白與凝膠之間有很好地相互作用；
5）適合低壓環(huán)境下的純化過(guò)程（大規(guī)模純化制備或預(yù)裝柱均可使用）；
6）高性價(jià)比，為研發(fā)、生產(chǎn)降低成本；
7）適用于大腸桿菌和真核細(xì)胞的裂解液和上清液；
8）同時(shí)可裝載Co, Cu, Zn和其它金屬離子。
優(yōu)勢(shì)詳解：
1. 收率高，純度高
Fig. 1: Over 20% more yield obtained with PureCube Ni-NTA Agarose. SDS-PAGE of GFP expressed in E. coli and purified in gravity columns with PureCube Ni-NTA Agarose and Ni-NTA resin from Competitor Q. 80 mg/mL protein yield was obtained with PureCube Ni-NTA Agarose (E1–E4, Cube) compared to 65 and 48 mg/mL, respectively, with the widely used alternative resins G and Q (E1–E4, Competitor G / Competitor Q).
Cube Biotech | |
蛋白結(jié)合能力 | > 80mg/mL |
2. *的DTT和EDTA穩(wěn)定性
Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.
PureCube Ni-NTA瓊脂糖在DTT和EDTA存在下非常堅(jiān)實(shí)，在穩(wěn)定性測(cè)試中，將PureCube Ni-NTA瓊脂糖置于DTT與EDTA濃度逐漸升高的環(huán)境中處理1小時(shí)。再用處理過(guò)的凝膠在重力層析柱中純化大腸桿菌表達(dá)的GFP-His，凝膠的蛋白結(jié)合能力因存在DTT與EDTA而下降，但是衰減率平緩，即使在1.5 mM EDTA，凝膠依然具有其結(jié)合能力的54%。
Cube Biotech | |
DTT穩(wěn)定性 | <10mM |
EDTA穩(wěn)定性 | <1.5mM |
產(chǎn)品特性：
應(yīng)用 | Specific binding and purification of 6x his-tagged proteins |
特異性 | Affinity to His-tagged proteins |
結(jié)合能力 | >80?mg/mL |
Bead Ligand | Ni-NTA |
粒徑大小 | 40 μm |
螯合劑穩(wěn)定性 | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
保存 | Delivered as a 50?% suspension |
所需試劑耗材 | Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50?mL tube (min 10,000 x g) 50?mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml ?pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment |
訂貨信息：
31101 | PureCube Ni-NTA Agarose (1 ml) |
31103 | PureCube Ni-NTA Agarose (10 ml) |
31105 | PureCube Ni-NTA Agarose (50 ml) |
31110 | PureCube Ni-NTA Agarose (250 ml) |
31112 | PureCube Ni-NTA Agarose (500 ml) |
31115 | PureCube Ni-NTA Agarose (1000 ml) |
31120 | PureCube Ni-NTA Agarose (5000 ml) |
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成都正民德思生物科技有限公司致力于成為中國(guó)與世界生物技術(shù)產(chǎn)業(yè)的紐帶，以務(wù)實(shí)的原則將歐洲高科技、高質(zhì)量、高效能的產(chǎn)品引進(jìn)中國(guó)。正民德思從2013年起至今，作為生物制藥和體外診斷領(lǐng)域相關(guān)產(chǎn)品的供應(yīng)商，秉承“為客戶增值"的企業(yè)價(jià)值觀提供優(yōu)質(zhì)的服務(wù)，通過(guò)對(duì)合作伙伴、客戶及社會(huì)的契約責(zé)任的高度重視，已積累與中國(guó)、德國(guó)、西班牙、奧地利、美國(guó)、芬蘭等眾多生命科學(xué)企業(yè)、生物技術(shù)公司、制藥公司、科研院校的合作經(jīng)驗(yàn)，并獲得合作伙伴和客戶的高度認(rèn)可并保持穩(wěn)固的戰(zhàn)略合作關(guān)系，互相支持、共同發(fā)展。
目前，成都正民德思生物科技有限公司作為德國(guó)蛋白純化產(chǎn)品生產(chǎn)商/服務(wù)商Cube Biotech生物科技公司中國(guó)總代理、西班牙生物磁性分離設(shè)備制造商SEPMAG中國(guó)總代理、西班牙免疫診斷試劑研發(fā)服務(wù)商Pragmatic Diagnostics公司的中國(guó)戰(zhàn)略合作方，在成都成立Cube Biotech、SEPMAG和Pragmatic Diagnostics的中國(guó)總部辦事處，全權(quán)負(fù)責(zé)在中國(guó)地區(qū)的營(yíng)銷事務(wù)、銷售及售后服務(wù)等所有工作。