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Source

Mouse

Applications

WB,IP,IF

Reactivity

Species independent

Dilution

WB: 1:3000; IP: 1:200; IF: 1:1000

Storage

-20°C/1 year

Specificity

The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.

Source/Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen

Synthetic peptide:HHHHHH conjugated to KLH.

Form

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Monoclonal

Background

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Concentration

1 mg/ml