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Source

Rabbit

Applications

WB,ELISA

Reactivity

Species independent

Dilution

WB: 1:1000 - 3000; ELISA: 1:20000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

DDDDK synthetic peptide conjugated to KLH.

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.

Clonality

Polyclonal

Background

The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Concentration

1 mg/ml