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Source

Rabbit

Applications

WB,ELISA

Reactivity

Species independent

Dilution

WB: 1:1000 - 3000; ELISA: 1:20000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

6X His synthetic peptide conjugated to KLH.

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.

Clonality

Polyclonal

Background

The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Concentration

1 mg/ml