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目錄:上海貝博生物科技有限公司>>Tag Antibodies>> MBP-Tag rabbit pAb

MBP-Tag rabbit pAb
  • MBP-Tag rabbit pAb
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更新時間:2024-11-11 18:26:35瀏覽次數:93評價

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  • Source

    Rabbit

  • Applications

    WB

  • Reactivity

    Species independent

  • Dilution

    WB: 1:5000

  • Storage

    -20°C/1 year

  • Specificity

    The antibody detects MBP & MBP tag fusion proteins.

  • Source/Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

  • Immunogen

    Recombinant Protein of MBP-Tag

  • Form

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Clonality

    Polyclonal

  • Background

    Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose.

  • Concentration

    1 mg/ml

Product Reactivity Applications Conjugation Catalog Images

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