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上海北諾生物科技有限公司

中級會員·13年

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15800960770

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中級會員·13年
聯(lián)人:
周經(jīng)理 劉經(jīng)理
話:
021-57730393
機(jī):
15800960770
真:
86-021-61496710
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上海市徐匯區(qū)宜山路520號中華門大廈18樓D座
化:
www.bnbiotech.com
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【DNA含量測定】 測序通用引物

2013-9-4  閱讀(4346)

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相關(guān)專題
引物設(shè)計
測序通用引物

載體 名稱 上游引物 5'primer 下游引物( 3'primer
P3*FLAG-CMV CMV-30(825-854) CMV-24(1129-1152)
pAc5.1/V5-His(_A,_B,_C) pAc5-5 BGH rev
pAcG2T pGEX-5 not available
pACT T7EEV T3/EBVrev
pACT2 GAL4 ADfor pGAL4-ADrv
pACT2 p17110 p12584
pACYC184(BamHI-site) PBRforBam PBRrevBam
pACYCDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1
pACYCDuet-1(MCSII) DuetUp2 T7-Terminator
pAdEasy-1 not available not available
pAdTrack-CMV not available not available
pAS2-1 GAL4 Bdfor/P24990 pGAL4-BDrv/P31430
pB42AD pB42ADF PB42ADR
pBABE pBMN_5'  
pBacPAK8 BAC 1 BAC2
pBAD pBAD-F pBAD-R
pBAD/HisABC pBAD-5'  
pBBR1MCS T3/M13rev T7/M13for
pBD-GAL4 pBD-GAL4-F PBD-GAL4-R
pBI121 35S NOS
pBIND GAL4-Bdfor T3,EBVrev
pBK-CMV M13F/T7 M13R/T3
pBluescriptIIKS(+/-) T3/M13rev T7/M13for(優(yōu)先用M13F)
pBluescriptIISK(+/-) T3/M13rev T7/M13for
pBluescriptKS(+/-) T3/M13rev T7/M13for
pBluescriptSK(+/-) T3/M13rev T7/M13for
pBR322(BamHI-site) PBRforBam PBRrevBam
pBR322(EcoRI/HindIII-sites) pBRforEco pBRrevHind
pBR325(BamHI-site) PBRforBam PBRrevBam
pBR329(BamHI-site) PBRforBam PBRrevBam
pBridge(MCSI) GAL4-Bdfor 3'BD
pBridge(MCSII) not available not available
pBS-T II T3/M13rev T7/M13for
pBS(AMP) T7 T3
pBSR T3 T7ter
pBudCE4.1(MCSI) CMV-Profor,T7 c-mycrev,EBVrev
pBudCE4.1(MCSII) EF-1α Fwd BGH
pBudCE4.1/lacZ/CAT CMV-Profor,T7 c-mycrev,EBVrev
pBV220 pBV220F PBV220R
pCAL-c T7/Tac-Profor T7-Terminator
pCAL-n T7/Tac-Profor T7-Terminator
pCAMBIA1301(1300) pCAMBIA1301-5'(ECORI)/M13R pCAMBIA1301-3'(HindIII)
pCAMBIA1304 pCAMBIA1301-5'(ECORI) pCAMBIA1301-3'(HindIII)
pCAMBIA2300 M13R(-48) M13F(-47)/M13F(-49)
pCANTB5E S1 S6
pCAT3-enhancer RVP3 此載體無反向引物
pCDFDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1
pCDFDuet-1(MCSII) DuetUP2 T7TER
pcDNA1.1 CMV-Profor,T7 pCDM8-3
pcDNA2.1 M13for/T7 M13rev/Tac-Profor
pcDNA3 T7/CMV-Profor BGH rev/SP6
pcDNA3.1(+/-) pcDNA3.1F/T7/CMV-Profor BGH rev
pcDNA3.1/His(_A,_B,_C) T7/CMV-Profor BGH rev
pcDNA3.1/myc-His(_A,_B,_C) T7/CMV-Profor BGH rev/c-mycrev
pcDNA3.1/V5-His(_A,_B,_C) T7/CMV-Profor BGH rev/V5rev
pcDNA3.1/Hygro(+,-) T7/CMV-Profor BGH rev/V5rev
pcDNA3.1/Zeo(+.-) T7 BGHrev
pcDNA3.3TOPOTA CMV-Profor TKPAreverse
pcDNA4/HisMax(_A,_B,_C) Xpressfor BGH rev
pcDNA4/HisMax-TOPO Xpressfor BGH rev
pcDNA4/HisMax-TOPO/lacZ Xpressfor BGH rev
pcDNA4/myc-His(_A,_B,_C) T7,CMV-Profor BGH rev,c-mycrev
pcDNA4/TO TREfor/CMV-Profor BGH rev
pcDNA4/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev
pcDNA4-TET/ON/AMP+ CMV-Profor  
pcDNA5/FRT T7/CMV-Profor BGH rev
pcDNA6/myc-His(_A,_B,_C) CMV-Profor,T7 BGH rev,c-mycrev
pcDNA6/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev
pCEP4 pCEP-F/CMV-Profor EBVrev
pCI T7(17BASE) EBVrev
pCI-neo T7(17BASE) EBVrev,T3
pCMS-EGFP T7 T3
pCMS-EGFP T7 EBVrev,T3
pCMV-3Tag-4A T3 /PFLAG-CMV-F T7
pCMV5 pCMV5F PCMV5R
pCMV6 CMV-Profor pCMV6-3、HGHrev
pCMV6-XL4 CMV-Profor/T7 pCMV6-3、HGHrev
pCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R,EBVrev
pCMV-MYC(AMP+) PCMV-F PCMV-R, EBVrev
pCMVmyc/nuc CMV-Profor BGH rev,c-mycrev
pcmv-scriptEXVector T3 T7
pCMV-Sport6 T7/M13for SP6/M13rev
pCOLADuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1
pCOLADuet-1(MCSII) DuetUP2 T7TER
pCR2.1 T7,M13for M13rev
pCR2.1-TOPO M13for,T7 M13rev
pCR3.1 T7 BGH rev
pCR3.1-Uni T7 BGH rev
pCR4 T3/M13rev T7/M13for
pCR4-Blunt T3/M13rev T7/M13for
pCR4-TOPO M13rev,T3 M13for,T7
pCR-Blunt M13rev T7,M13for
pCR-BluntII M13rev/SP6 T7/M13for
pCR-BluntII-TOPO M13rev/SP6 M13for/T7
pCRII M13rev/SP6 T7/M13for
pCRII-TOPO M13rev/SP6 M13for/T7
pCR-ScriptSK(+) T7/M13for T3/M13rev
pCR-XL-TOPO M13rev T7,M13for
pCS2+(MCSI) SP6 EBVrev,T7?
pCS2+(MCSII) T3 pSG5-3
pDB_GAL4cam T7P  
pDB_leu PDBLeu-5 PDBLeu-3
pDEST22 DEST22F DEST22R
pDEST15 pGEX-5 T7-Terminator
pDEST20 pGEX-5 pFastBac-3
pDEST26 TREfor/CMV-Profor T7/M13for
pDisplay T7/CMV-Profor c-mycrev
pDNR-LIB(MCS_A) M13for,T7 pSG5-3,M13R
pDonar M13F M13R
pDONR201 PDONR-F PDONR-R
pDONR207 PDONR-F PDONR-R
pDONR223 M13F M13R
pDrive T7/M13rev SP6/M13for
pDsRED1-C1 (DsRed1-N-f) DsRed1-C-r
pDsRed1-N1 CMV-Profor RFP-Nrev,CMV-R?
pDsRed2-N1 (DsRed1-N-f) DsRed1-C-r
pDsRed2-C1 (DsRed1-N-f) DsRed1-C-r
pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’
pDsRed-Express dsRed1_N_primer/CMV-F dsRed1_C_primer/CMV-R
pDSRED-monomer-N1(KAN+) dsRed1_N_primer  
pDSRED-N1(KAN+) pEGFP-N-5’ pDSRED-N-R
pEASY-Blunt M13F T7/ M13R
pEASY-BluntSimple M13F T7/ M13R(距離插入位點(diǎn)合適,優(yōu)先安排)
pEASY-T1 Simple M13F M13R(距離插入位點(diǎn)合適,優(yōu)先安排)
pEASY-T1 M13F/T7 M13R
pEASY-T3 M13F/T7 M13R/SP6
pECFP-N(C) PECFP-N-5. PECFP-N-3,
pEF/myc/cyto/ER/mito/nuc pEF-5 Pcdna3.1R/BGH rev
pEF1(4,6/myc-His) T7/pEF-5 Pcdna3.1R/BGH rev
pEF1(4,6/V5-His) T7/pEF-5 Pcdna3.1R/BGH rev
pEF1(4;6/His_A;_B;_C) T7/Xpressfor Pcdna3.1R/BGH rev
pEF5/FRT/V5-D(-TOPO) T7/pEF-5 Pcdna3.1R/BGH rev
pEGFP M13rev EGFP-Nrev
pEGFP-1 not available EGFP-Nrev/ PEGFP-N-3′
pEGFP-C PEGFP-C-5′ PEGFP-C-3′
pEGFP-C1 EGFP-Cfor SV40-pArev
pEGFP-C2 EGFP-Cfor SV40-pArev
pEGFP-C3 EGFP-Cfor SV40-pArev
pEGFP-F EGFP-Cfor SV40-pArev
pEGFP-N PEGFP-N-5′ PEGFP-N-3′
pEGFP-N1 CMV-Profor EGFP-Nrev
pEGFP-N2 CMV-Profor EGFP-Nrev
pEGFP-N3 CMV-Profor EGFP-Nrev
pENTR M13F M13R
pET-11(-a,-b,-c,-d) T7 T7-Terminator
pET-12(-a,-b,-c) T7 T7-Terminator
pET-14b T7 T7-Terminator
pET-15b T7 T7-Terminator
pET-16b T7 T7-Terminator
pET-17b T7 T7-Terminator
pET-17xb T7 T7-Terminator
pET-19b T7 T7-Terminator
pET-20b(+) T7 T7-Terminator
pET-21(-a,-b,-c,-d)(+) T7 T7-Terminator
pET-22b(+) T7 T7-Terminator
pET-23(-a,-b,-c,-d)(+) T7 T7-Terminator
pET-24(-a,-b,-c,-d)(+) T7 T7-Terminator
pET-25b(+) T7 T7-Terminator
pET-26b(+) T7 T7-Terminator
pET-27b(+) T7 T7-Terminator
pET-28a(-b,-c)(+) T7 T7-Terminator
pET-29a(-b,-c)(+) S.tag/T7 T7-Terminator
pET-3(-a,-b,-c,-d) T7 T7-Terminator
pET-30_Ek/LIC T7 T7-Terminator
pET-30_Xa/LIC T7 T7-Terminator
pET-30a(-b,-c)(+) S.tag/T7 T7-Terminator
pET-31b(+) not available T7-Terminator
pET-32_Ek/LIC TRXfor T7-Terminator
pET-32_XA/LIC TRXfor T7-Terminator
pET-32a(-b,-c)(+) S.tag/TRXfor T7-Terminator
pET-33b(+) T7 T7-Terminator
pET-34b(+) S.tag T7-Terminator
pET-35b(+) S.tag T7-Terminator
pET-36b(+) S.tag T7-Terminator
pET-37b(+) S.tag T7-Terminator
pET-38b(+) not available T7-Terminator
pET-39b(+) S.tag T7-Terminator
pET-40b(+) S.tag T7-Terminator
pET-41_Ek/LIC GST-Cfor T7-Terminator
pET-41a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator
pET-42a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator
pET-43.1a(-b,-c)(+) S.tag coliDOWN
pET-43.1a_EK/LIC S.tag not available
pET-44a(-b,-c)(+) S.tag T7-Terminator
pET-44a_EK/LIC S.tag not available
pET-45b(+) T7 T7-Terminator
pET-46_Ek/LIC T7 T7-Terminator
pET-5(-a,-b,-c) T7 T7-Terminator
pET-52b(+) T7 T7-Terminator
pET-7 T7 T7-Terminator
pET-9(-a,-b,-c,-d) T7 T7-Terminator
pETcoco-1 T7 T7-Terminator
pETDuet-1(MCSI) pETUP1 DuetDOWN1
pETDuet-1(MCSII) DuetUp2 T7-Terminator
pET-T4 T7 T7-Terminator
pEYFP-C1 pEGFP-C-5’ pEGFP-C-3'
pEYFP-N1 pEGFP-N-5’,CMV-Profor pEGFP-N-3’
pFLAG-ATS N26for pTrcHis-rv;C24rev
pFLAG-CTC N26for pTrcHis-rv;C24rev
pFLAG1 N26for pTrcHis-rv;C24rev
pFLAG-CMV(-2) CMV-30;CMV-Profor CMV-24
pFLAG-CMV1 CMV-30 CMV-24
pGAD424 GAL4_ADfor 3'AD
pGADGH GAL4_ADfor 3'AD
pGADT7 T7/GAL4_ADfor 3'AD
pGADT7-Rec T7/GAL4_ADfor 3'AD
pGADT-T T7 3,BD
pGAD10 GAL4_ADfor 3'AD
pGAPZa-A a-FACTOR 3'AOX
pGBKT7 T7 3,BD
pGBT9 MATCHMAKER5'DNA-BD/GAL4BD MATCHMAKER3'DNA-BD
pGEM-1 T7 SP6
pGEM-3 T7 SP6
pGEM-3Zf(+)(-) SP6/M13rev T7/M13for
pGEM-4 T7 SP6
pGEM-4Z T7/M13for SP6/M13rev
pGEM-5Zf(+)(-) SP6/M13rev T7/M13for
pGEM-7Zf(+)(-) SP6/M13rev T7/M13for
pGEM-9Zf(+)(-) T7/M13for SP6/M13rev
pGEM-T T7/M13for SP6/M13rev
pGEM-T_Easy T7/M13for SP6/M13rev
pGene/V5-His_A(_B,_C) pGENE-5 BGH rev,V5rev
pGEX-2T pGEX-5 pGEX-3
pGEX-2TK pGEX-5 pGEX-3
pGEX-3X pGEX-5 pGEX-3
pGEX-4T-1(-2,-3) pGEX-5 pGEX-3
pGEX-5X-1(-2,-3) pGEX-5 pGEX-3
pGEX-6P-1(-2,-3) pGEX-5 pGEX-3
pGL2 GLP1 GLP2
pGL3 RVP3 GLP2(RVP4)(沒有特別說明用GLP2)
pGL3-Basic(MCSI) RVP3(PGL3+)? GLP2(PGL3-)?
pGL3-Basic(MCSII) RVP4, PLXSN-3'?
pGL4    
pGlow(-TOPO) T7 pGlow-3
pGM-T T7 SP6
pGWC SP6 T7
pHook-2 T7/CMV-Profor not available
pIND/V5-His_A(_B,_C) not available BGH rev,V5rev
PinpointTMVector PinpointPrimer SP6
pIRES(MCSI) T7 pIRES-3
pIRES(MCSII) not available T3,EBVrev
pIRES2-EGFP CMV-Profor,pIRES2-EGFP.P5’ IRESrev,pIRES2-EGFP.P3’
pIRES-hrGFP-2a CMV-Profor,T3 pIRES-hrGFP-3
pIRESneo2 T7/CMV-Profor pIRES-3
pIVEX(all) T7/pBRrevBam T7-Terminator
pLEXA pLEXA-F pLEXA-R
pLNCX pLNCX-F pLNCX-R
pLNCX2 CMV-Profor pRevTRE-3
pLVTHM(H1啟動子下游) H1primer SP6
pLXSN pLXSN-F pLXSN-R
pMALc MalE primer M13F(-47)
pMAL-c2E MalE primer M13F(-47)
pMAL-C2x Pmal-c2x-F(P5’) Pmal-C2X-R(P3’ )
pMALd MalE primer M13F(-47)
pMALe MalE primer M13F(-47)
pMAL-P4X MalE primer M13F(-47)
pMAL-p2X MalE primer M13F(-47)
pMET A/B/C AUG1F AUG1R
pMD18-T M13R(-48)ECORI M13F(-47)(HINDⅢ)
pMD19-T M13F47 M13R48
pMD20-T M13F47 M13R48
pMOSBlue T7/M13rev M13for
pMSCV-puro pLXSNfw pMSCVrev
pMT/V5-His_A(_B,_C) Mtfor BGH rev,V5rev
pNTAP T3 T7
pCTAP pCTAP5'primer T7
pOTB7 M13rev/T7 M13for/SP6
pOptiVEC-TOPO TA CMV-F/CMV-Profor EMCVIRES reverse
pPC86 PPC86-F PPC86-R
pPC97 PBD-GAL4-F PBD-GAL4-R
pPIC3.5K 5'AOX 3'AOX
pPic9k(A+,Z+) 5’AOX(Ppic9k-f)/a-factor 3’AOX(Ppic9k-R)
pPICZa 5'AOX/PPICZa-F 3'AOX
pPROEXHTA M13R(-48) 無反向引物
pProEX-HTa(b,c) M13R48/ Tac-Profor pBADrv/pTrcHis-3
pPD129.36   M13F20/M13F
pQE30or40or60(AMP+) PQE30-F PQE-30R
pRC/CMV2/CAT CMV/T7  
pRECEIVER CMV-F 此載體 無反向引物
pRECEIVER-M03 pRECEIVER-M03F pRECEIVER-M03R
pREP4 pREP4 forward primer EBV reverse primer
pRK5 SP6 pSG5-3
pRSET T7/Xpressfor T7ter
pRSFDuet-1(MCSI) DuetUP1 DuetDOWN1
pRSFDuet-1(MCSII) DuetUP2 T7TER
pSecTac2 T7/CMV-Profor BGH rev/c-mycrev
pSG5 pSG5-5,/T7 PSG5-R(SV40)
pSHlox1 T7 SP6
pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R
pSI T7 EBVrev
pSilencer1.0-U6 T7 T3
pSilencer2.0-U6 T7 M13for
pSilencer2.1-U6neo T7 M13for
pSilencer3.1-H1hygro M13F(-47) 3.0REV
pSilencer4_1-CMVneo pSilener4.1-5' CMV-Profor
pSILENCEV2.0-U6 T7 2.0REV
pSIneo T7 EBVrev,T3
Psit T7 T7T
pSK01-T M13F M13R
pSK-CMV(KAN+) T7 T3
pSOS Psos5' Psos3'
pSP64 SP6 M13rev
pSP64/65 SP6 此載體無反向引物
pSP65 SP6 M13rev
pSP70 SP6 T7
pSP71 SP6 T7
pSP72 SP6 T7
pSP73 SP6 T7
pSport1 SP6/M13F(-47) T7/M13R
pSPORT1 SP6/M13for T7/M13rev
pSPT18 SP6 T7
pSTBlue T7/M13rev SP6/M13for
pSUPER.Basic T7/M13F M13R
pSUPER T7 T3
pSURE-T M13F M13R
pSURE-Tsimple M13F-47 M13R-48
pSuperCos pBRforEco T7
pT3T7 M13rev/T3 M13for/T7
pT7-7 T7 not available
pT7Blue® T7 M13F(-47)
pT7T3_18U M13rev/T7 M13for/T3
pT7T3D-PAC M13rev/T7 M13for/T3
pTA2 M13F/T7(優(yōu)先使用M13F) T3/M13R
pT-Adv M13rev T7,M13for
pTAg T7/M13rev M13for
pTARGETTM pTarget.F(在T7前面)/T7 pTarget.R
pTG19-T M13F(-47) M13R(-48)
pTARGETTM T7/M13F PTARGET-SEQ
pThioHisA,B,andC Trx Forward Trx Reverse / M13for
pTO-T7 T7 T7TER
pTRC99A PTRC99A-F pTrcHis-rv
pTrc99a-c PTRC99C-F PTRC99C-R
pTRC99a-c PTRC99C-F PTRC99C-R
pTrcHisB PTrcHisA-F/Xpressfor PTrcHisA-R
pTrcHisA PTrcHisA-F PTrcHisA-R
pT-REX-DEST30 TREfor T7
pTriEx1 T7/TriExup TriExdown
pTriEx1.1 T7/TriExup TriExdown
pTriEx1.1_Hygro T7/TriExup not available
pTriEx1.1_Neo T7/TriExup IRESrev
pTriplEX1 TriplEx-LD-5 T7,TriplEx-LD-3
pTriplEX2 TriplEx-LD-5;pTR5‘ T7,TriplEx-LD-3
pTWIN-1 T7 T7TER
pTXB1 T7 MxeInteinrev
pTXB2 T7 MxeInteinrev
pTXB3 T7 MxeInteinrev
pTYB1 T7 Inteinrev
pTYB2 T7 Inteinrev
pTYB3 T7 Inteinrev
pTYB4 T7 Inteinrev
pTZ_18U T7/M13rev M13for
pUB6/V5-His UB-F BGH rev,V5rev
pUC13 M13rev/M13rev(-48) M13for/M13for(-49)
pUC18 M13rev/M13rev(-48) M13for/M13for(-49)
pUC18(19)/118(119) M13F(-47) M13R(-48)/PUC19(HINDⅢ)
pUC19 M13rev/M13rev(-48) M13for/M13for(-49)
pUC57 M13rev/M13rev(-48) M13for/M13for(-49)
pUC8 M13rev/M13rev(-48) M13for/M13for(-49)
pUC9 M13rev/M13rev(-48) M13for/M13for(-49)
pUM-T M13F(-20) M13R(-20)
pUCm-T(AMP+) M13F/T7 M13R
pUNI/V5-His not available BGH rev,V5rev
pVAX1T7 T7/CMV-Profor PCDNA3.1R/BGHR
pVP22/mycHis VP22-Cfor BGH rev,c-mycrev
pVP22/mycHis2 T7/CMV-Profor VP22-Nrev
pWE15 pBRrevHind T7
pYES2 T7 pYes2.R
pYES-DEST52 T7/GAL1-Profor V5rev/CYC1-Terminator
pYEX_4T_1 Clontech pYEX_4T-3
pYEX_4T_2 pGEX-5 pYEX_4T-4
pYEX_4T_3 pGEX-5 pYEX_4T-4
pYEX_4T_4 pGEX-5 pYEX_4T-4
pZeoSV2+/hu-Tyr T7 not available
pZERO-1 M13rev/SP6 T7/M13for
pZERO-2 M13rev/SP6 M13for/T7
pZome-1-C Zome-F Zome-R
pZome-1-N Zome-F Zome-R
YCp50(BamHI-site) PBRforBam PBRrevBam
YEp13(BamHI-site) PBRforBam PBRrevBam
YEp24(BamHI-site) PBRforBam PBRrevBam
YIp5(BamHI-site) PBRforBam PBRrevBam
RNAi -ReadypSIREN-RetroQ-U6ZsGreen U6  
菌PCDB T7 BGH
天為時代TA載體 T7 T3
m13(+) T7 T3
pMSCV-neo pMSCV-5' pMSCV-3'

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