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25006620 RPN3682 CDP-STAR

CDP-Star Detection Reagent

• Fast and convenient system for all high-sensitivity applications using DNA, RNA or oligonucleotide probes.
• Hybridization stringency can be controlled by elevating temperatures up to 75°C.
• Detection of 60 fg target DNA （single-copy gene） in a genomic Southern blot.
• ECF™ detection for quantitation experiments.
• Saves 2 to 3 h over conventional indirect methods.

AlkPhos Direct™ Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used.

DNA or RNA probes are labeled during a 30 min reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration. Detection is rapid, taking only 30 min from completion of hybridization to application of detection reagent.

Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm² or 5000 cm² membrane （depending on product ordered）: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star™ or ECF™ detection reagents.