The TRIM24 TR-FRET Assay Kit is designed to measure the inhibition of TRIM24 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no timeconsuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbiumlabeled donor, dye-labeled acceptor, TRIM24, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.