目錄:MedChemExpress LLC>>生化試劑>> BCECF | MCE
CAS | 85138-49-4 | 分子量 | 520.44 |
---|---|---|---|
分子式 | C??H??O?? | 供貨周期 | 現貨 |
貨號 | HY-101882 | 應用領域 | 醫療衛生,化工,生物產業,制藥 |
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CAS No. : 85138-49-4
產品活性:BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm. Storage: protect from light.
研究領域:Others
作用靶點:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Prepare a 2-20 mM stock solution of BCECF in DMSO and store at -20℃. 2. Prepare a 5-50 µM BCECF dye-loading solution in buffer solutions (Hepes, pH 7.4 or PBS).3. Add 1000 µL/well (6-well plate),100 µL/well (96-well plate) or 25 µL/well (384-well plate) BCECF dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator about 30minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements.
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