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應用領域	生物產業

Flexcell細胞加壓培養系統

美國flexcell:體外細胞應力刺激培養金標準

l 歷史悠久、成功使用案例和文獻多、無技術和使用風險：30多年歷史，6000+文獻，1300+實驗室使用，國內200+單位使用

l 真空動力柔性基底膜細胞應力應變和三維細胞接種凝膠應力應變

l 不同基底剛度牽張：2019年研制了基底剛度1-60kPa可調控牽張拉伸培養皿和多孔板。

l 加載力學類型全面：牽張、壓力、流體剪切應力

l 受力均勻、培養效果好：真空氣體牽張、壓縮刺激方式

l 不同基底剛度模量力學環境裝置的廠家，2019進一步研制了 “更軟"基底剛度可調節可牽張培養裝置：

0.1-80kPa可調控牽張拉伸培養皿和多孔板，避免細胞在體外培養受到“硬度"沖擊

l 模擬任意波形：動靜、靜態以及自定義波形

l 細胞量大，便于后期分析：支持四個24孔或6孔牽張拉伸

l 即用型國際標準化的各種蛋白預先包被應力刺激培養耗材

l 適用各種形態細胞組織樣品：單層細胞、3D細胞構建體、組織或3D聚集體

l 多模態刺激：靜態刺激、周期性動態刺激、動靜態對照、單軸向或雙軸向（等邊軸）牽張刺激

l 的可以動靜態對照的廠家：可使同一塊壓力或牽張培養板部分孔受力。

l 的倒置和正置顯微鏡系統都兼容的廠家：受力同時可實時觀察

l 整合柔性基底拓撲微圖案和微通道, 方便高級實驗模型設計

l 整合力電刺激偶聯，方便高級實驗模型設計

l 牽張、流體剪切多力混合同時加載，方便高級實驗模型設計

l 使用Flexcell® Transwell Holder三維細胞（或者）拉力或壓力共培養

l 設備模塊化設計牽張、壓縮、流體剪切可以任意組合，緊湊大氣。

以下是一些有關細胞力學培養的文獻推薦：

- Bougault C, Aubert-Foucher E, Paumier A, Perrier-Groult E, Huot L, Hot D, Duterque-Coquillaud M, Mallein-Gerin F. Dynamic compression of chondrocyte-agarose constructs reveals new candidate mechanosensitive genes. PLoS One 7(5):e36964,2012.

- Bougault C, Paumier A, Aubert-Foucher E, Mallein-Gerin F. Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression. BMC Biotechnol 8:71,2008.

- Chen X, Guo J, Yuan Y, Sun Z, Chen B, Tong X, Zhang L, Shen C, Zou J. Cyclic compression stimulates osteoblast differentiation via activation of the Wnt/β-catenin signaling pathway. Molecular Medicine Reports 15(5):2890-2896, 2017.

- Damaraju S, Matyas JR, Rancourt DE, Duncan NA. The effect of mechanical stimulation on mineralization in differentiating osteoblasts in Collagen-I scaffolds. Tissue Eng Part A 20(23-24):3142-3153, 2014.

- Damaraju S, Matyas JR, Rancourt DE, Duncan NA. The role of gap junctions and mechanical loading on mineral formation in a Collagen-I scaffold seeded with osteoprogenitor cells. Tissue Eng Part A 21(9-10):1720-32, 2015.

- Fermor B, Haribabu B, Weinberg JB, Pisetsky, Guilak F. Mechanical stress and nitric oxide influence leukotriene production in cartilage. Biochemical and Biophysical Research Communications 285:806–810,2001.

- Fermor B, Weinberg JB, Pisetsky DS, Guilak F. The influence of oxygen tension on the induction of the nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage. Osteoarthritis Cartilage 13:935-941,2005.

- Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Banes AJ, Guilak F. The effects of static and intermittent compression on nitric oxide production in articular cartilage explants. J Orthop Res 9(4):729-737, 2001.

- Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Fink C, Guilak F. Induction of cyclooxygenase-2 by mechanical stress through a nitric oxide-regulated pathway. Osteoarthritis Cartilage 10:792–798,2002.

- Fink C, Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Guilak F. The effect of dynamic mechanical compression on nitric oxide production in the meniscus. Osteoarthritis Cartilage 9(5):481-487, 2001.

- Fox DB, Cook JL, Kuroki K, Cockrell M. Effects of dynamic compressive load on Collagen-based scaffolds seeded with Fibroblast-like synoviocytes. Tissue Eng 12(6):1527-1537, 2006.

- Glaeser JD, Salehi K, Kanim LE, NaPier Z, Kropf MA, Cuellar J, Sheyn D, Bae HW. Treatment with the NF?B inhibitor reduces overloading-induced MMP expression in human nucleus pulposus cells. The Spine Journal 17(10):S127,2017.

- Gosset M, Berenbaum F, Levy A, Pigenet A, Thirion S, Saffar JL, Jacques C. Prostaglandin E2 synthesis in cartilage explants under compression: mPGES-1 is a mechanosensitive gene. Arthritis Research & Therapy 8:R135,2006.

- Graff RD, Lazarowski ER, Banes AJ, Lee GM. ATP release by mechanically loaded porcine chondrons in pellet culture. Arthritis Rheum 43(7):1571-1579, 2000.