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PC-12細胞

細胞名稱： 大鼠腎上腺嗜鉻細胞瘤細胞(未分化)

細胞別名：PC-12; PC 12

貨號：TCR-C617

規格： 1X10^6

價格：1500 元（推薦T25瓶，復蘇發貨1-2周）

PC-12細胞細胞系來自可移植的雄性大鼠的腎上腺嗜鉻細胞瘤；表達神經生長因子(NGF)受體，NGF可誘導該細胞產生可逆性的神經表型。該細胞可產生兒茶酚胺、多巴胺和去甲腎上腺素，但不合成腎上腺素。

種屬來源：大鼠

組織來源：腎上腺

疾病特征：嗜鉻細胞瘤

細胞形態：圓形

生長特性：疏松貼壁和懸浮同時存在

PC-12細胞培養體系：RPMI-1640+5%FBS+15%馬血清+1%P/S

配套培養基貨號：TCR-G617

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存

質量檢測：細菌、真菌、支原體檢測均為陰性

參考文獻：

Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897