近日，北京理工大學(xué)生命學(xué)院高海·軍課題組劉鑫老師，使用IPHASE品牌產(chǎn)品：NADPH再生系統(tǒng)在《Microbial Cell Factories》權(quán)·威期刊上發(fā)表文章《Engineering cascade biocatalysis in whole cells for syringic acid bioproduction》，影響因子6.4！
丁香酸(Syringic acid, SA)是一種具有多種生物活性的高價值天然化合物，廣泛存在于水果、蔬菜和草藥中。SA主要是通過化學(xué)合成來生產(chǎn)的，然而這些化學(xué)方法有許多缺點，如設(shè)備要求高、反應(yīng)條件苛刻、催化劑昂貴、副產(chǎn)品眾多等。因此，在本研究中，設(shè)計并開發(fā)了一種新的利用工程全細(xì)胞生產(chǎn)SA的生物轉(zhuǎn)化途徑。
研究設(shè)計了一個多酶級聯(lián)體系，在大腸桿菌中利用靜息或生長的全細(xì)胞合成SA。本工作鑒定了一種O-甲基化酶(DesAOMT)，它可以催化GA甲基化生成SA。含4種酶的多酶級聯(lián)反應(yīng)在工程大腸桿菌中表達。菌株的代謝系統(tǒng)和生物轉(zhuǎn)化條件影響催化效率。本研究為SA生物合成提供了一條新的綠色途徑。
摘要
Background Syringic acid (SA) is a high-value natural compound with diverse biological activities and wide applications, commonly found in fruits, vegetables, and herbs. SA is primarily produced through chemical synthesis, nonetheless, these chemical methods have many drawbacks, such as considerable equipment requirements, harsh reaction conditions, expensive catalysts, and numerous by-products. Therefore, in this study, a novel biotransformation route for SA production was designed and developed by using engineered whole cells.
Results An O-methyltransferase from Desulfuromonas acetoxidans (DesAOMT), which preferentially catalyzes a methyl transfer reaction on the meta-hydroxyl group of catechol analogues, was identifed. The whole cells expressing DesAOMT can transform gallic acid (GA) into SA when S-adenosyl methionine (SAM) is used as a methyl donor. We constructed a multi-enzyme cascade reaction in Escherichia coli, containing an endogenous shikimate kinase (AroL) and a chorismate lyase (UbiC), along with a p-hydroxybenzoate hydroxylase mutant (PobA**) from Pseudomonas fuorescens, and DesAOMT; SA was biosynthesized from shikimic acid (SHA) by using whole cells catalysis. The metabolic system of chassis cells also afected the efciency of SA biosynthesis, blocking the chorismate metabolism pathway improved SA production. When the supply of the cofactor NADPH was optimized, the titer of SA reached 133 μM (26.2 mg/L).
Conclusion Overall, we designed a multi-enzyme cascade in E. coli for SA biosynthesis by using resting or growing whole cells. This work identifed an O-methyltransferase (DesAOMT), which can catalyze the methylation of GA to produce SA. The multi-enzyme cascade containing four enzymesexpressed in an engineered E. coli for synthesizing of SA from SHA. The metabolic system of the strain and biotransformation conditions infuenced catalytic efciency. This study provides a new green route for SA biosynthesis.
完整版文獻可在【IPHASE】公眾號后臺留言【獲取文章】
再次恭喜文獻發(fā)表，以及對我司產(chǎn)品的認(rèn)可，希望以上文獻能幫助大家了解目前研究進展及我們的核心技術(shù)，歡迎各位新老客戶聯(lián)系我們咨詢、提出意見，愿我們的努力成果與您的科研碰撞出不一樣的火花！
發(fā) 文 章 得 獎 勵
凡使用本公司產(chǎn)品，在國內(nèi)及國際刊物上發(fā)表論文(論文發(fā)表日起一年內(nèi))，并注明產(chǎn)品屬于IPHASE BIOSCIENCES Co.,Ltd. / 匯智和源生物技術(shù)(蘇州)有限公司所有，即可申請獎勵。根據(jù)發(fā)表刊物影響因子不同，給予不同金額獎品：
非SCI論文及IF≤5分，500元禮品；
5分
8分
IF≥10分 2000元禮品；
注：①禮品卡也可兌換同等金額產(chǎn)品購買抵用券；
②如遇我司公司名稱書寫不規(guī)范或不是第一作者
等情況，對應(yīng)給予獎品金額將發(fā)放50%；
活動多多，禮品豐厚，快來參與吧！
關(guān) 于 我 們
匯智和源，致力于為創(chuàng)新藥研發(fā)企業(yè)及生命科學(xué)研究機構(gòu)提供高品質(zhì)的生物試劑，IPHASE為公司核心品牌，品牌宗旨“Innovative Reagents For Innovative Research”。
6