8當前位置：> 供求商機> RIG-I Knockout - Reporter Human Lung Carcinoma
當前位置：> 供求商機> RIG-I Knockout - Reporter Human Lung Carcinoma
當前位置：> 供求商機> RIG-I Knockout - Reporter Human Lung Carcinoma
Product Name：RIG-I Knockout - Reporter Human Lung Carcinoma
Cat：a549d-korigi-cov2r
Cell Line Stability
Cells undergo genotypic changes resulting in reduced responsivenessover time in normal cell culture conditions. Genetic instability is abiological phenomenon that occurs in all stably transfected cells.Therefore, it is critical to prepare an adequate number of frozen stocksat early passages. A549-Dual™ KO-RIG-I hACE2-TMPRSS2 cells shouldnot be passaged more than 20 times to remain fully efficient.
Quality Control
• The overexpression of the human ACE2 (hACE2) gene has beenverified by RT-qPCR, FACS staining, and functional assays.
• The overexpression of the human TMPRSS2 (hTMPRSS2) gene hasbeen verified by RT-qPCR and functional assays.
• RIG-I KO has been verified by (RT-q)PCR and functional assays.
• Lucia luciferase and SEAP reporter activities have been validatedusing functional assays.
• The stability for 20 passages following thawing has been verified
• These cells are guaranteed mycoplasma-free.
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