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當前位置：> 供求商機> 190101—磁性固定化金屬親和層析填料
Product IntroductionImmobilised metal affinity chromatography (IMAC) has been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing histidine (or cysteine and tryptophane). The presence of several adjacent histidines such as (His)6-tag increases the affinity to immobilised metal ions. Increasingly, IMAC resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells. There are other applications of IMAC resins to purification of certain native non-tagged proteins as well, such as interferons, lectins, antibodies, serum and plasma proteins, peptides and peptide hormones.
Product Properties:
Four types of IMAC SepFast MAG are supplied in 5 ml format with precharged metal ions as Ni SepFast MAG, Cu SepFast MAG, Zn SepFast MAG and Co SepFast MAG. A special pack that contains the above four pre-charged resins (1 ml each type) is supplied as well for the screening purpose.
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