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廣州市錦卓儀器設(shè)備有限公司
主營產(chǎn)品： HLB固相萃取小柱,S-x3聚苯乙烯凝膠填料,SPME（固相微萃取頭） |
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廣州市錦卓儀器設(shè)備有限公司
主營產(chǎn)品： HLB固相萃取小柱,S-x3聚苯乙烯凝膠填料,SPME（固相微萃取頭） |
聯(lián)系電話
| 參考價(jià) | ￥ 4400 |
| 訂貨量 | 1 |
更新時(shí)間：2024-09-11 16:26:28瀏覽次數(shù)：4027
聯(lián)系我們時(shí)請(qǐng)說明是化工儀器網(wǎng)上看到的信息，謝謝!
| 供貨周期 | 現(xiàn)貨 | 貨號(hào) | 556547 |
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FITC膜聯(lián)蛋白V染色先于膜完整性的喪失，伴隨著由凋亡或壞死過程引起的細(xì)胞死亡階段。因此，F(xiàn)ITC膜聯(lián)蛋白V的染色通常與重要染料（如碘化丙啶（PI）或7-氨基放線菌素（7-AAD））結(jié)合使用，以使研究人員能夠鑒定早期凋亡細(xì)胞（PI陰性，F(xiàn)ITC膜聯(lián)蛋白V正）。具有完整膜的活細(xì)胞排除PI，使死亡和受損細(xì)胞的膜可透過PI。例如，認(rèn)為可行的細(xì)胞是FITC膜聯(lián)蛋白V和PI陰性; 早期凋亡細(xì)胞是FITC Annexin V陽性和PI陰性; 細(xì)胞凋亡或已經(jīng)死亡的細(xì)胞都是FITC膜聯(lián)蛋白V和PI陽性。該測(cè)定不區(qū)分已經(jīng)經(jīng)歷凋亡死亡的細(xì)胞與由于壞死性途徑死亡的細(xì)胞，因?yàn)樵谌我磺闆r下，死細(xì)胞都會(huì)用FITC Annexin V和PI染色。然而，隨著時(shí)間的推移，當(dāng)細(xì)胞凋亡被測(cè)量時(shí)，細(xì)胞可以經(jīng)常從FITC膜聯(lián)蛋白V和PI陰性（可行或無可測(cè)量的凋亡）追溯到FITC膜聯(lián)蛋白V陽性和PI陰性（早期凋亡，膜完整性存在），zui后到FITC膜聯(lián)蛋白V和PI陽性（末期凋亡和死亡）。細(xì)胞通過這三個(gè)階段的運(yùn)動(dòng)表明細(xì)胞凋亡。相比之下，一個(gè)單一的觀察結(jié)果表明，細(xì)胞都是FITC膜聯(lián)蛋白V和PI陽性，本身就顯示出關(guān)于細(xì)胞進(jìn)行死亡的過程的信息較少。
在4°C下未經(jīng)稀釋保存，防止長時(shí)間暴露于光線下。不要凍結(jié)
Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, FITC Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation.
FITC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with FITC Annexin V is typically used in conjunction with a vital dye such as propidium iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (PI negative, FITC Annexin V positive). Viable cells with intact membranes exclude PI, wheras the membranes of dead and damaged cells are permeable to PI. For example, cells that are considered viable are FITC Annexin V and PI negative; cells that are in early apoptosis are FITC Annexin V positive and PI negative; and cells that are in late apoptosis or already dead are are both FITC Annexin V and PI positive. This assay does not distinguish between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because in either case, the dead cells will stain with both FITC Annexin V and PI. However, when apoptosis is measured over time, cells can be often tracked from FITC Annexin V and PI negative (viable, or no measurable apoptosis), to FITC Annexin V positive and PI negative (early apoptosis, membrane integrity is present) and finally to FITC Annexin V and PI positive (end stage apoptosis and death). The movement of cells through these three stages suggests apoptosis. In contrast, a single observation indicating that cells are both FITC Annexin V and PI positive, in of itself, reveals less information about the process by which the cells underwent their demise.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
