當前位置:深圳欣博盛生物科技有限公司>>核苷酸>> Biotin-16-Aminoallyl-2'-dCTP - (N-5002)
供貨周期 | 一個月 | 貨號 | N-5002 |
---|---|---|---|
應用領域 | 生物產業,綜合 |
Biotin-16-Aminoallyl-2'-dCTP - (N-5002)又名Biotin-16-dCTP, Biotin-16-AA-dCTP。
產品描述
Biotin-16-Aminoallyl-2'-deoxycytidine-5'-Triphosphate可用于通過PCR、切口平移、使用Klenow(exo-)DNA聚合酶的引物延伸和末端脫氧核苷酸轉移酶標記的3'端,將生物素摻入DNA中。可以使用Moloney鼠白血病病毒(M-MLV)逆轉錄酶與此dNTP制備生物素化的cDNA。
產品信息
貨號 | N-5002 | 規格 | 50 nmole,1 µmole |
純度 | ≥90% by AX-HPLC | 保存溫度 | -20℃或以下 |
分子式 | C32H53N8O17P3S (free acid) | 鹽離子形式 | Li+ |
消光系數 | 5,040 Lmol-1cm-1 at 290 nm | 溶液 | H2O |
濃度 | 5 mM | 骨架 | 5'-三磷酸鹽 |
糖類型 | DNA | 核苷酸類別 | 生物素標記 |
應用 | 適體、表觀遺傳學/DNA損傷、體外轉錄、誘變、光交聯研究 |
文獻參考:
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Veneziano, Rémi; Shepherd, Tyson R.; Ratanalert, Sakul; Bellou, Leila; Tao, Chaoqun; Bathe, Mark . In vitro synthesis of gene-length single-stranded DNA.
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Ho, Nicholas R. Y.; Lim, Geok Soon; Sundah, Noah R.; Lim, Diana; Loh, Tze Ping; Shao, Huilin . Visual and modular detection of pathogen nucleic acids with enzyme-DNA molecular complexes.
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