The J116 monoclonal antibody reacts with human PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1's structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments. Binding of the J116 antibody is reported to inhibit PD-1 signal transduction, however, it is not reported to block PD-L1 binding.

產(chǎn)品名稱:InVivoMab anti-human PD-1 (CD279)

反應(yīng)種屬:human

宿主來(lái)源:mouse

實(shí)驗(yàn)應(yīng)用:in vitro PD-1 neutralization, in vivo PD-1 blockade in humanized mice

同種型:IgG1, κ

克隆性:Monoclonal

克隆號(hào):J116

產(chǎn)品濃度:Lot specific,typically between 4-11 mg/ml

保存溫度:Undiluted at 4°C in the dark

運(yùn)輸溫度:Ship with adequate amount of blue ice to ensure the product remains at 4°C for the duration of transit

預(yù)期分子量:150 kDa

產(chǎn)品形式:液體

有效期:One year from the date of receipt if stored as recommended

產(chǎn)地:美國(guó)

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