 | 紅榮微再（上海）生物工程技術有限公司 |
15021010459
在線溝通：
顏先生 （銷售）
- 電話：
- 400-021-2200
- 手機：
- 15021010459
- 傳真：
- 聯系我時，
- 告知來自化工儀器網
- 個性化：
- www.hyrmb.com.cn
- 公司網站：
- www.hyrmb.com
[供應]BioLife CryoStor CS5 細胞DMSO凍存液現貨
CryoStor CS5細胞凍存液特點
用于細胞和組織的高級生物保存液
預混DMSO
無血清、無蛋白
USP級高質量成分、cGMP級生產、FDA master文件
滅菌、內毒素和細胞功能檢測
BioLife Solutions 細胞凍存液 CryoStor CS5 Freeze Media GMP級DMSO
產品英文名 BioLife Solutions CryoStor CS5 Freeze Media
產品中文名 BioLife Solutions CryoStor CS5細胞DMSO凍存液
產品介紹 BioLife Solutions CryoStor®是BioLife公司特別優化的細胞凍存液，在極低溫度 (-70ºC to -196ºC)下準備和保存細胞。預混DMSO，為細胞和組織的冷凍、儲存和解凍過程提供安全的保護環境。CryoStor®通過調控凍存過程的分子生物學反應，無血清、蛋白或具有高細胞毒性的試劑，就能增強細胞活力和功能。
產品訂購信息
品牌 | 貨號 | 產品描述 | 包裝 |
BioLife Solutions | 205373 | CryoStor CS5 Freeze Media 細胞DMSO凍存液 | 5×10mL |
BioLife Solutions | 205102 | CryoStor CS5 Freeze Media 細胞DMSO凍存液 | 100 mL/瓶 |
BioLife Solutions | 205202 | CryoStor CS5 Freeze Media 細胞DMSO凍存液 | 100 mL/袋 |
BioLife Solutions CryoStor CS5 Freeze Media GMP級細胞凍存液205373 205102 205202現貨供應歡迎您咨詢華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司
CryoStor CS5細胞凍存液特點
用于細胞和組織的高級生物保存液
預混DMSO
無血清、無蛋白
USP級高質量成分、cGMP級生產、FDA master文件
滅菌、內毒素和細胞功能檢測
DMSO無血清細胞凍存液適用細胞
多種類型細胞，包括如肝細胞之類的敏感細胞
BioLife Solutions CryoStor CS5 細胞DMSO凍存液cGMP級生產
MATERIALS ARE MANUFACTURED UNDER cGMP
CryoStor® Usage and Cryopreservation Protocol使用與凍存方法
細胞準備
1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)
2) Centrifuge cells to obtain cell pellet
3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.
加CryoStor凍存液
4) ISOLATION: Add cold (2-8°C) CryoStor®
a. Cell concentrations: 0.5-10 x 106cells/ml for routine cell culture protocols (higher [cell] possible).
b. DMSO is pre-mixed in CryoStor® - no additives are necessary.
5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes
6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)
a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.
b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.
c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.
d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.
儲存
7) STORAGE: Place samples into storage
a. Store samples at liquid nitrogen temperatures (below -130°C).
b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).
細胞復蘇解凍
8) THAWING: Thaw samples quickly in a 37°C water bath
a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.
b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.Passive thaw is not recommended.
9) Dilute cell/CryoStor® mixture immediay with culture media
a. Dilution procedure can be preformed in a single step.
b. The dilution media should be between 20°C and 37°C.
c. A dilution ratio of 1:10 (sample to media) or greater is recommended.
10) Plate cells in appropriate configuration
11) Place cells into culture conditions or utilize immediay
12) Viability assessment 24-hours post-thaw*
Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.
*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.
Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.
Visual inspection of adherent cells and cells “floating” in the media is also recommended.
BioLife Solutions CryoStor CS5/CS10 Freeze Media 細胞凍存液
品牌 | 貨號 | 產品描述 | 包裝 |
BioLife Solutions | 210102 | CryoStor CS10 Freeze Media 細胞DMSO凍存液
| 100 mL/瓶 |
BioLife Solutions | 210210 | CryoStor CS10 Freeze Media 細胞DMSO凍存液 | 1000 mL/袋 |
BioLife Solutions | 210202 | CryoStor CS10 Freeze Media 細胞DMSO凍存液 | 100 mL/袋 |
BioLife Solutions | 210374 | CryoStor CS10 Freeze Media 細胞DMSO凍存液 | 16 mL/瓶 |
BioLife Solutions | 210373 | CryoStor CS10 Freeze Media 細胞DMSO凍存液 | 10 mL/瓶 |
BioLife Solutions | 205373 | CryoStor CS5 Freeze Media 細胞DMSO凍存液 | 5×10mL |
BioLife Solutions | 205102 | CryoStor CS5 Freeze Media 細胞DMSO凍存液 | 100 mL/瓶 |
BioLife Solutions | 205202 | CryoStor CS5 Freeze Media 細胞DMSO凍存液 | 100 mL/袋 |
CS5和CS10主要區別是規格包裝不同。
BioLife Solutions CryoStor CS5 Freeze Media GMP級細胞凍存液205373 205102 205202現貨供應歡迎您咨詢華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司歡迎您咨詢華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司 ：1500 1904 520。紅榮微再-客服: 2395557778 經銷商專員
以上信息由企業自行提供，信息內容的真實性、準確性和合法性由相關企業負責，化工儀器網對此不承擔任何保證責任。 溫馨提示：為規避購買風險，建議您在購買產品前務必確認供應商資質及產品質量。
