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[供應(yīng)]PeproTech 大血管內(nèi)皮細(xì)胞培養(yǎng)基 PeproGrow 700-MacroV
產(chǎn)地:美國(guó)
更新時(shí)間:2024-10-17 14:21:59
有效期:2024年10月17日 -- 2025年4月18日
已獲點(diǎn)擊:251
PeproGrow MacroV 大血管內(nèi)皮細(xì)胞培養(yǎng)基適用于培養(yǎng)大血管內(nèi)皮細(xì)胞。能良好維持內(nèi)皮祖細(xì)胞的形態(tài)和功能,并提高特定標(biāo)志物—內(nèi)皮一氧化氮合酶活性。
PeproGrow™ MacroV (Macrovascular Endothelial Cell Media)
英文名:PeproGrow™ MacroV (Macrovascular Endothelial Cell Media)
中文名:PeproGrow™ MacroV 大血管內(nèi)皮細(xì)胞培養(yǎng)基
PeproGrow MacroV 大血管內(nèi)皮細(xì)胞培養(yǎng)基適用于培養(yǎng)大血管內(nèi)皮細(xì)胞。能良好維持內(nèi)皮祖細(xì)胞的形態(tài)和功能,并提高特定標(biāo)志物—內(nèi)皮一氧化氮合酶活性。
概述
PeproGrow™ MacroV 大血管內(nèi)皮細(xì)胞培養(yǎng)基
Product Details
Catalog Number:700-MacroV
Description:
PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro c*tion of: endothelial progenitor cells (EPC; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPC; growing cells at rates that exceed commercially available media. These media formulations are optimized for primary/progenitor human cells, but can also be used for bovine, porcine, rabbit, rat, and mouse EPC, or mature endothelial cells.
PeproGrow MacroV is supplied as a 500mL bottle of basal medium and a separate, frozen growth factor component.
PeproGrow MacroV內(nèi)皮細(xì)胞培養(yǎng)基適用細(xì)胞:
PeproGrow MacroV is recommended for Macrovascular Endothelial Cells:
Human Umbilical Vein Endothelial Cells (HUVEC) 人臍靜脈內(nèi)皮細(xì)胞
Human Umbilical Artery Endothelial Cells (HUAEC) 人臍動(dòng)脈內(nèi)皮細(xì)胞
Human Aortic Endothelial Cells (HAoEC) 人大動(dòng)脈內(nèi)皮細(xì)胞
Human Pulmonary Artery Endothelial Cells (HPAEC) 人肺動(dòng)脈內(nèi)皮細(xì)胞
Human Saphenous Vein Endothelial Cells (HSaVEC) 人隱靜脈內(nèi)皮細(xì)胞
And other large vessel (macrovascular) endothelial cells 以及其他大血管內(nèi)皮細(xì)胞
PeproGrow MacroV內(nèi)皮細(xì)胞培養(yǎng)基用于人臍靜脈內(nèi)皮細(xì)胞擴(kuò)增的性能
Figure 1: HUVEC Proliferation. HUVEC were seeded onto fibronectin-coated plates and incubated for 7 days in PeproGrow MacroV basal medium + Growth Supplement MacroV and two competitors' medium. The proliferation ability of HUVEC was assessed 7 days after cell c*tion using the XTT assay following the manufacturer’s protocol. The proliferation ability of HUVEC was expressed as the average of fluorescence intensity (O.D.) calculated using a plate reader from two independent assays run in triplicates.
人臍靜脈內(nèi)皮細(xì)胞擴(kuò)增
PeproTech 內(nèi)皮細(xì)胞培養(yǎng)基系列
PeproTech提供三種內(nèi)皮細(xì)胞培養(yǎng)基試劑盒,分別適用于體外培養(yǎng):骨髓或外周血來(lái)源的內(nèi)皮祖細(xì)胞(PeproGrow™ EPC ),大血管內(nèi)皮細(xì)胞(PeproGrow™ MacroV)和微血管內(nèi)皮細(xì)胞(PeproGrow™ MicroV)。每款試劑盒由基礎(chǔ)培養(yǎng)基和一管單獨(dú)的內(nèi)皮細(xì)胞生長(zhǎng)添加劑組成。
品牌 | 貨號(hào) | 產(chǎn)品描述 | 包裝 |
PeproTech | 700-EPC | PeproGrow™ EPC (Endothelial Progenitor Cell Media)內(nèi)皮祖細(xì)胞培養(yǎng)基試劑盒 | 1 Kit |
PeproTech | 700-MacroV | PeproGrow™ MacroV (Macrovascular Endothelial Cell Media)大血管內(nèi)皮細(xì)胞培養(yǎng)基試劑盒 | 1 Kit |
PeproTech | 700-MicroV | PeproGrow™ MicroV (Microvascular Endothelial Cell Media)微血管內(nèi)皮細(xì)胞培養(yǎng)基試劑盒 | 1 Kit |
其他內(nèi)皮細(xì)胞培養(yǎng)基
ScienCell 1001 內(nèi)皮細(xì)胞培養(yǎng)基 現(xiàn)貨供應(yīng)
歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 :1500 1904 520。紅榮微再-客服: 2395557778 經(jīng)銷商專員
背景——人臍靜脈內(nèi)皮細(xì)胞
來(lái)源:Wiki
Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis).[1] They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth. HUVECs were first isolated and cultured in vitro in the 1970s by Jaffe and others. [2] HUVECs can be easily made to proliferate in a laboratory setting. Like human umbilical artery endothelial cells they exhibit a cobblestone phenotype when lining vessel walls.
人臍靜脈內(nèi)皮細(xì)胞來(lái)自臍帶血管的內(nèi)皮細(xì)胞。因?yàn)閮r(jià)格低和分離技術(shù)簡(jiǎn)單,人臍靜脈內(nèi)皮細(xì)胞通常作為內(nèi)皮細(xì)胞的功能和生理研究的實(shí)驗(yàn)?zāi)P褪褂?。人臍靜脈內(nèi)皮細(xì)胞是在1970年由Jaffe等人*分離使用的。在實(shí)驗(yàn)條件下人臍靜脈內(nèi)皮細(xì)胞很容易擴(kuò)增。
人臍靜脈內(nèi)皮細(xì)胞(HUVEC)獲取
來(lái)源:互聯(lián)網(wǎng)
1. 將 15-20cm 長(zhǎng)的新生兒臍帶放入無(wú)菌的 PBS 溶液中儲(chǔ)存。(注:4℃下zui多貯存 24 小時(shí),室溫下不超過(guò) 6 小時(shí),否則廢棄)
2. 用一個(gè)鈍頭的針頭扎入臍帶靜脈管中,用無(wú)菌的 PBS 溶液沖洗 3-5 次,將污血沖洗干凈為止。
3. 用手術(shù)鉗夾緊臍帶下端,加入 15ml 的膠原酶(1mg/ml)室溫下消化 15-20分鐘,并不時(shí)上下?lián)u動(dòng)臍帶。
4. 消化完后,將下端手術(shù)鉗松開,消化液流入一個(gè) 50 ml 無(wú)菌離心管中,用無(wú)菌的 PBS 溶液沖洗臍帶 2-3 次。
5. 將收集液離心(2000 轉(zhuǎn)/ 分)3 分鐘。
6. 倒去上清。加培養(yǎng)基。
歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 :1500 1904 520。紅榮微再-客服: 2395557778 經(jīng)銷商專員
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