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PicoPLEX WGA Kit 單細胞全基因組擴增試劑盒常見問題
閱讀：2062發布時間：2017-11-6
1.推薦加樣量
1-10個人類細胞（如：卵裂球，極體，滋胚層細胞，羊水細胞，循環腫瘤細胞，培養細胞）
1000-10000個細菌細胞
不完整或片段化，單/雙鏈DNA
2.PicoPLEX® 單細胞全基因組擴增試劑盒推薦應用？
PicoPLEX單細胞全基因組擴增試劑盒用于：
CNV拷貝數變異：寡核苷酸和細菌人工染色體的微陣列比較基因組雜交（oligonucleotide and BAC aCGHa）
單核苷酸多態性分型SNP
PCR法基因突變篩查
3.使用PicoPLEX WGA Kit前，可以用什么緩沖液洗滌單細胞？
一些客戶成功使用加了PVA（聚乙烯醇，例：0.1%）的PBS洗滌收集細胞。另外，注意洗滌/收集緩沖液（PBS）不要含鈣、鎂離子。
4.流式分選有什么特殊要求嗎？
Yes, we strongly recommend not fixing, and using light scattering or phase contrast to sort or collect samples. Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions.
5.PicoPLEX WGA Kit的加樣體積？
每次反應5 μl 加樣體積（細胞和緩沖液總體積不超過2.5 μl）。
6.單細胞全基因組擴增產物能直接用于NGS嗎？
不能。
3.那么有其他能直接應用NGS的單細胞全基因組擴增試劑盒嗎？
有。PicoPLEX DNA-seq直接應用Illumina NGS。
8.What is the expected genomic locus representation and Loss of Heterozygosity (LOH) for PicoPLEX WGA products?
For reference purposes, see the data obtained from a third party in the table below.
SNP Genotyping Method | Single-Cell Amplification Success Rate | SNP Call Rates | LOH |
PCR | 95% | > 95% | < 10% |
Illumina Infinium SNP array | 95% | 50% – 60% | 7% – 12% |
9.單細胞全基因組擴增技術的終產物是單鏈還是雙鏈DNA？
產物是單雙鏈DNA的混合物。
PicoPLEX WGA Kit 單細胞全基因組擴增試劑盒問答英文版
1. What are the recommended DNA inputs for the PicoPLEX WGA Kit?
1 – 10 human cells (e.g. blastomeres, polar bodies, trophoblastic cells, amniocytes, CTCs, cultured cells
1,000 – 10,000 bacterial cells
Intact or fragmented, single- or double-stranded DNA
2.What applications are recommended for PicoPLEX® WGA Kit?
This kit is used for:
Copy number variation (CNV) analysis: superior for oligonucleotide and BAC aCGH（Bacterial Artificial Chromosome Array-based comparative genomic hybridization）
SNP genotyping
Mutation detection by PCR
3. Is there a non-stick wash buffer that can be used for single cell wash prior to using PicoPLEX WGA Kit?
This has not been validated at Rubicon Genomics, but some customers have successfully used PVA (Polyvinyl Alcohol; e.g. 0.1%) in PBS to wash/collect their cells. Additionally, your washing/collecting buffer (PBS) must be also Ca- and Mg-free.
4. Are there special requirements for flow sorting?
Yes, we strongly recommend not fixing, and using light scattering or phase contrast to sort or collect samples. Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions.
5. What is the sample input volume for PicoPLEX WGA Kit?
This kit will accommodate a 5 μl sample input volume per reaction (with a maximum of 2.5 μl of cell & buffer carryover).
6. Can the product of PicoPLEX WGA Kit be taken straight to NGS?
No.
7. Does Rubicon Genomics have an NGS-ready kit for single cell applications?
Yes, PicoPLEX DNA-seq can be used with Illumina NGS platforms.
8. Sigma-Aldrich sells a GenomePlex WGA kit, is it the same technology as PicoPLEX WGA?
No, PicoPLEX uses different technology.
9. What is the expected genomic locus representation and Loss of Heterozygosity (LOH) for PicoPLEX WGA products?
For reference purposes, see the data obtained from a third party in the table below.
SNP Genotyping Method | Single-Cell Amplification Success Rate | SNP Call Rates | LOH |
PCR | 95% | > 95% | < 10% |
Illumina Infinium SNP array | 95% | 50% – 60% | 7% – 12% |
10. Is the product of this technology single- or double-stranded?
The end product of PicoPLEX WGA is a mixture of single- and double-stranded DNA.
PicoPLEX WGA Kit 單細胞全基因組擴增試劑盒問答英文版摘自Rubicon，中文版由紅榮微再翻譯整理。
>>>>>>>>>>>>>>>>>>>>>>>>>>訂購直達的分割線>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
選購RB3050 Rubicon PicoPLEX WGA Kit 單細胞全基因組擴增試劑盒產品歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司 ：152 1681 4001。華雅再生醫學-客服: 316 808 6348。
RB3050 Rubicon PicoPLEX WGA Kit 單細胞全基因組擴增試劑盒
PicoPLEX WGA Kit采用技術，設計優化用于從單細胞中擴增全基因組DNA。起始樣本量低至單細胞或6pg基因組DNA。便捷的單管操作流程降低污染節省時間。結果穩定性、重復性好。
PicoPLEX WGA Kit適用array CGH分析和PCR平臺。PicoPLEX技術被IVF供應商如BlueGnome信賴并用于PGS，BlueGnome CGH也是中國福利會和平婦幼保健院所用的PGS平臺。
產品訂購信息
貨號 | 產品名稱 | 包裝 |
RB3050/R30050 | PicoPLEX WGA Kit單細胞全基因組擴增試劑盒，array CGH、PCR分析用 | 50次反應 |
RB3381/R300381 | PicoPLEX DNA-seq Kit，48種雙端illumina引物 | 48 |
紅榮微再（上海）生物工程技術有限公司以“傳遞科學價值，服務科學研究”為宗旨，主營：干細胞、醫療、細胞治療、器官再生 四大板塊的產品。
紅榮微再醫療宣傳冊
選購RB3050 Rubicon PicoPLEX WGA Kit 單細胞全基因組擴增試劑盒產品歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司 ：152 1681 4001。華雅再生醫學-客服: 316 808 6348。
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