ATP4b抗體

抗原
ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b)
別名 ATP6B, AV080843, S76401S1
抗原表位
其他選擇 Middle Region
N-Term(8),AA 60-90 (7),C-Term (4),AA 1-291 (3),AA 52-78 (2),AA 67-176 (1),AA 97-291 (1),Internal Region (1),Middle Region (1),Subunit beta (1)
適用
其他選擇 Rabbit, Murine, Rat ((Rattus)), Dog (Canine), Bovine, Pig (Porcine), Xenopus laevis, Zebrafish (Danio rerio), Human, Chicken
Human(44),Murine (23),Rat ((Rattus)) (21),Bovine (15),Dog (Canine) (15),Pig (Porcine) (15),Chicken (13),Ferret (1),Rabbit (1)
宿主
其他選擇 Rabbit
Rabbit(40),Mouse (7)
克隆類型
多克隆
標記
其他選擇 非結合性
Biotin(3),FITC (3),HRP (3),PE (2),APC (1),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1)
應用范圍
其他選擇 Western Blotting (WB)
Western Blotting (WB)(37),Flow Cytometry (FACS) (21),ELISA (19),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5),Immunoassay (IA) (3),Immunohistochemistry (IHC) (3),Immunoprecipitation (IP) (3),Immunofluorescence (IF) (2),Immunohistochemistry (Frozen Sections) (IHC (fro)) (2),Chromatin Immunoprecipitation (ChIP) (1),Immunocytochemistry (ICC) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1)
[Pubmed] 1 reference available
規格 50 μg
發貨至 中國 (更改)
發貨時間 9至12個工作日

ATP4b抗體

產品細節

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產品細節

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免疫原 Synthetic peptide directed towards the middle region of human ATP4B
序列 QVKYYPPNGT FSLHYFPYYG KKAQPHYSNP LVAAKLLNIP RNAEVAIVCK
預測反應 Bovine : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Chicken : 90 %
產品特性 This is a rabbit polyclonal antibody against ATP4B. It was validated on Western Blot using a cell lysate as a positive control.
純化方法 Affinity Purified

目標詳細情況

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目標詳細情況

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別名 ATP4B
背景 ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
分子量 33 kDa
基因ID 496
NCBI Accession NP_000696, NM_000705
UniProt P51164
研究領域 Enzymes, Metabolism

使用細節

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使用細節

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應用備注 Optimal working dilutions should be determined experimentally by the investigator.
說明

Antigen size: 291 AA

限制 For Research Use only

貯存及處理

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貯存及處理

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狀態 Lyophilized
溶解方式 Add 50 μL of distilled water.
濃度 1 mg/mL
緩沖液 PBS buffer with 2 % sucrose
注意事項 Avoid repeated freeze-thaw cycles.
儲存條件 -20 °C
儲存方法 For longer periods of storage, store at -20 °C

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