英文名稱 BrdU
中文名稱 溴脫氧尿苷抗體
別    名 Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker.
溴脫氧尿苷抗體
  
說 明 書 0.1ml  0.2ml  
產品類型 藥物與化合物抗體 
研究領域 細胞生物  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 BrdU
產品應用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 20/48kDa
細胞定位 細胞核 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 BrdU conjugated to KLH
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

產品介紹 background:
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.
BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Subcellular Location:
Nuclear.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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