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南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
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南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
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更新時間：2023-07-24 13:42:41瀏覽次數：601
聯系我們時請說明是化工儀器網上看到的信息，謝謝!
| 供貨周期 | 現貨 | 規格 | T-25 Flask |
|---|---|---|---|
| 貨號 | CBP74170 | 應用領域 | 生物產業 |
| CBP74170 | |
| I. Background | |
The particles contain a firefly luciferase gene driven by the human IL-2 promoter. After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity. IL-15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL-1beta, IFN-gamma, or TNF- alpha and may thus play a role in T-cell mediated immune responses in the human central nervous system. IL-15 stimulates proliferation of the established T-cell line CTLL-2, CD8(+) memory T- cells require IL-15 for proliferation. IL-15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL-15 appears to function as a specific maturation factor for NK-cells and can mimic the bone marrow microenvironment in vitro, which is required for NK- cells to acquire lytic functions. IL-15 induces proliferation of mast cells in the absence of functional IL-2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T-cell IL-15 receptors. The IL-15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3, and STAT3 and STAT5 that are activated in T-cells. The alpha subunit of the IL-15 receptor (IL-15RA) is structurally related to the alpha subunit of the IL-2 receptor and confers high affinity binding of IL-15 to its receptor. IL-15 binds to the beta and gamma chains of the IL2 receptor, which are known to be required for ligand internalization and signal transduction. | |
| II. Introduction | |
| Expressed gene: | IL2R、IL15R |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | IL15, AI503618, IL-15, interleukin 15, IL15, Il15 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+10ug/ml blasticidin+800ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
Figure 1. Recombinant hIL2/IL15 Dual Effector(hCD122/hCD132) Reporter Cell constitutively expressing IL2Rα.
Figure 2. Recombinant hIL2/IL15 Dual Effector(hCD122/hCD132) Reporter Cell constitutively expressing IL2Rβ.
Figure 3. Recombinant hIL2/IL15 Dual Effector(hCD122/hCD132) Reporter Cell constitutively expressing IL2Rγ.
Figure 4. Dose Response of Recombinant Human IL2/IL15 in hlL2/IL15 Dual Effector(hCD122/hCD132) Reporter Cells (C9). | |
