 |
南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
10
聯系電話
|
南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
聯系電話
| 參考價 | 面議 |
更新時間：2023-06-09 11:26:49瀏覽次數：684
聯系我們時請說明是化工儀器網上看到的信息，謝謝!
| 供貨周期 | 現貨 | 規格 | T-25 Flask |
|---|---|---|---|
| 貨號 | CBP74021 | 應用領域 | 生物產業 |
| 主要用途 | 僅限科研使用 |
TLR8/NFkB-Luc/HEK293
| CBP74021 | |
| I. Background | |
| The TLR family plays a fundamental role in pathogen recognition and activation of innate immunity. TLR8 is an endosomal receptor that recognizes single stranded RNA (ssRNA), and can recognize ssRNA viruses such as Influenza, HIV and HCV. TLR8 binding to the viral RNA recruits MyD88 and leads to activation of the transcription factor NFκB and an antiviral response. Human TLR8 is functional but murine TLR8 is non-functional because it lacks five amino acids. | |
| II. Description | |
Recombinant HEK293 cell expressing firefly luciferase gene under the control of NFkB response elements with constitutive expression of human TLR8 (Toll-like receptor 8, also known as CD288, a member of the toll-like receptor (TLR) family (GenBank Accession No. NM_138636). | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | TLR8、NFkB-Luc |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | CD288, TLR8, Toll-like receptor 8, tlr, tlr-8 TLR, nfkb, nf-kb reporter TLR |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | kidney |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). TLR8/NF-kB Reporter - HEK293 Recombinant Cell Line(C1).. | |
