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南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
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南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
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更新時間：2023-06-09 11:22:20瀏覽次數：811
聯系我們時請說明是化工儀器網上看到的信息，謝謝!
| 供貨周期 | 現貨 | 規格 | T-25 Flask |
|---|---|---|---|
| 貨號 | CBP74017 | 應用領域 | 生物產業 |
| 主要用途 | 僅限科研使用 |
OX40/NFkB-Luc/HEK293
| CBP74017 | |
| I. Background | |
| Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Its ligand, OX40L, binds to OX40 receptors on T-cells, preventing them from dying and subsequently increasing cytokine production. OX40 has a critical role in the maintenance of an immune response beyond the first few days and onwards to a memory response due to its ability to enhance survival. | |
| II. Description | |
| Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327) | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | NFκB-Luciferase-OX40 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
| |
Figure 1. Recombinant HEK293 cell expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327) | |
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Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). HEK293/NFκB Luciferase-OX40 Reporter cells were stimulated by OX40L, the EC50 was 82.7 ng/ml. | |
