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南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
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南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
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更新時間：2023-06-09 11:29:27瀏覽次數：877
聯系我們時請說明是化工儀器網上看到的信息，謝謝!
| CBP74045 | |
| I. Background | |
Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients. | |
| II. Description | |
Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry. | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | 4-1BB |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | 4-1BB, TNFRS9, Mitogen-Activated Protein Kinase 14, Signal Transducer And Activator Of Transcription 3, STAT3, MAPK14 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of 4-1BB. | |
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). 4-1BB/NF-κB Reporter-HEK293 cells were stimulated by 4-1BBL, the S/B was 14.1-fold. |
