 |
南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
10
聯系電話
|
南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
聯系電話
| 參考價 | 面議 |
更新時間：2022-04-29 10:06:55瀏覽次數：652
聯系我們時請說明是化工儀器網上看到的信息，謝謝!
| 供貨周期 | 現貨 | 規格 | T-25 Flask |
|---|---|---|---|
| 貨號 | CBP73283 | 應用領域 | 生物產業 |
| 主要用途 | 僅限科研使用 |
CBP73283 | |
| I. Introduction | |
Cell Line Name： | c-Kit W557_K558del-V654A/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
KIT W557_K558del results in the deletion of 2 amino acids in the juxtamembrane domain (exon 11) of the Kit protein from amino acids 557 to 558 (PMID: 12918066). W557_K558del results in constitutive activation of Kit and increased Erk phosphorylation, and leads to increased invasiveness and expression of ETV1 in cell culture (PMID: 25239608, PMID: 26936919). | |
| III. Representative Data | |
1.WB of c-Kit W557_K558del-V654A/BaF3
| |
2.Sanger of c-Kit W557_K558del-V654A/BaF3
Figure 2. c-Kit W557_K558del-V654A/BaF3 V654A
Figure 3. c-Kit W557_K558del-V654A/BaF3 W557_K558del | |
3. Anti-proliferation assay
Figure 4. CTG Proliferation Assay of BaF3 C-KIT W557_ K558del/V654A(C8). | |
| |
