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南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
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南京科佰生物科技有限公司
主營產品： atcc代理,ATCC細胞系,ATCC細胞株,ATCC細胞庫購買 |
聯系電話
| 參考價 | 面議 |
更新時間：2022-04-28 14:25:34瀏覽次數：591
聯系我們時請說明是化工儀器網上看到的信息，謝謝!
| 供貨周期 | 現貨 | 規格 | T-25 Flask |
|---|---|---|---|
| 貨號 | CBP73287 | 應用領域 | 生物產業 |
| 主要用途 | 僅限科研使用 |
| CBP73287 | |
| I. Introduction | |
Cell Line Name： | BCR-ABL1 [Y253H]/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
Presence of a BCR-ABL1 fusion gene is necessary for the pathogenesis of CML. In up to 95% of cases, a t(9;22) (q34;q11) translocation results in the BCR-ABL1 fusion gene (Faderl et al. 1999). This translocation results in the Philadephia chromosome. In rare CML cases lacking the traditional t(9;22) translocation, other translocations result in the creation of the BCR-ABL1 fusion gene, which sometimes involve multiple chromosomes. | |
| III. Representative Data | |
1. WB of BCR-ABL1 [Y253H]/BaF3
| |
2.Sanger of BCR-ABL1 [Y253H]/BaF3
Figure 2. BCR-ABL1 Y253H
Figure 3. BCR-ABL1 [Y253H]/BaF3 Fusion | |
3. Anti-proliferation assay
Figure 4. CTG Proliferation Assay of BaF3 BCR-ABL1 Y253H Cells (C1). | |
