詳細介紹
什么是親水作用色譜
親水作用色譜是一種直接分離極性和親水性化合物的色譜技術(shù),為了便于理解,我們可以把它看作為是一種用反相色譜溶劑做流動機動的正相色譜。
因此HILIC是:
•親水性的固定相
•以水、有機溶劑和緩沖鹽做為流動相洗脫劑
常見的HILIC方法采用50-90%有機溶劑和一定比例的水,并且含有低濃度的緩沖鹽。如:乙腈/水/醋酸銨體系。在HILIC方法中,洗脫順序和反相色譜正好相反,即極性越大,保留時間越長
產(chǎn)品目錄編號 | 150662 |
概述 | SeQuant® ZIC®-cHILIC is designed for improved separation of polar hydrophilic compounds that have proved difficult to separate by reversed-phase HPLC or previous types of HILIC columns. The orientation of its zwitterionic functional group provides more accessible positive charges, which results in a complementary selectivity with more retention particularly for organic acids and other negatively charged compounds. Especially suitable for LC-MS thanks to an extraordinary high column stability and a minimized column bleed. This long 250 mm column is suitable for very complex samples. |
HS代碼 | 9027 90 50 |
儲存類別 | 10 - 13 其它液體及固體 |
Pressure | ≤ 90 bar |
Theoretical Plates (N/m) (Cytosine) | ≥ 90000 |
Symmetry (Cytosine) | 0.95 - 1.25 |
產(chǎn)品料號 | 產(chǎn)品貨號 | *產(chǎn)品名稱 | *產(chǎn)品規(guī)格 |
ME1504790001 | 1.50479.0001 | ZIC-HILIC Capillary Columnn | SeQuant® ZIC®-HILIC (3.5µm,200Å) 150 x 0.3 mm |