HumanTh1/Th2/Th17 Phenotyping Kit
Product Information
Material Number: 560751
Size: 50 tests
Description
Components:
51-9006615 Human Th1/Th2/Th17 Phenotyping Cocktail 1.0 ml
Containing the following:

560750   HU T CELL ACT KIT 50TST
560751   HU TH1/2/17 PHNOTYP KIT 50TST
560752   HU TH1/17 PHNOTYP KIT 50TST
560753   HU REG TCELL SORT KIT
560754 Mouse CD335/Nkp 46 purified 29A1.4 0.5MG
Human CD4 PERCP-CY5.5 (clone: SK3)
Human IL-17A PE (clone: N49-653)
Human IFN-GMA FITC (clone: B27)
Human IL-4 APC (clone: MP4-25D2)
51-9006613 BD Cytofix™ Fixation Buffer 100 ml
51-2091KE BD Perm/Wash™ Buffer 25 ml
51-2092KZ BD GolgiStop™ Protein Transport Inhibitor (containing monensin) 0.7 ml
The peripheral CD4+ T cell pool includes multiple effector and memory T cell subsets that arise through antigen-driven
expansion and differentiation of naïve T cells. The early response of naïve CD4+ T cells to antigenic stimulation is characterized
by high level proliferation and a limited cytokine repertoire. Further differentiation yields cells with a more diverse potential for
cytokine expression. Depending upon the balance of local cytokines, costimulatory molecules, antigen levels, and genetic
factors, Type-1 T helper (Th1), Th2, and Th17 effector and/or memory cells are generated by immune responses.

外周血CD4+ T細胞池包括多個效應和記憶T細胞亞群的出現，通過抗原驅動的

膨脹和Naï幼稚T細胞的分化。的Naï已經CD4 + T細胞的抗原刺激的早期反應的特征

高水平的細胞因子的增殖和有限的劇目。進一步分化產生的細胞與不同的潛力

細胞因子的表達。根據局部細胞因子平衡，共刺激分子，抗原的水平，和遺傳

因素，Ⅰ型T輔助細胞（Th1，Th2和Th17細胞效應），和/或記憶細胞通過產生的免疫應答。

Functionally-polarized CD4+ T cell subsets have been identified based on their distinctive patterns of cytokine secretion. As a
signature cytokine, Th1 cells selectively produce large amounts of interferon-gamma (IFN-γ). Th2 cells selectively produce IL-4,
and Th17 express high levels of IL-17A. Through secretion of IFN-γ and other effector molecules, Th1 cells activate
macrophages, natural killer (NK) cells, and CD8+ T cells and are responsible for cell-mediated immunity. Th1 cells provide
protection against intracellular bacteria, fungi, protozoa and viruses and are involved in some autoimmune responses. IL-4
produced by Th2 cells is particularly strong in driving B cells to generate IgE-secreting cells. IgE plays a role in basophil/mast
cell mediated immune reactions. Th2 cells mediate protection against extracellular parasites but may also cause harmful allergic
responsiveness to develop. Through the secretion of IL-17A and other factors, Th17 cells recruit and activate neutrophils and
mediate immune responses against extracellular bacteria and fungi. Th17 cells are also implicated in autoimmune responses. In
addition to these types of T helper Investigators should note that the appearance of BD GolgiStop™ Protein Transport Inhibitor may range in color from clear
(colorless) to light yellow.

功能性極化的CD4 + T細胞亞群是基于細胞分泌細胞因子的*的模式識別。作為一個

簽名細胞因子，Th1細胞選擇性地產生大量的干擾素γ（IFN-γ）。Th2細胞產生IL-4的選擇性，

Th17細胞表達高水平的IFN-γIL-17A。通過與其他效應分子的分泌，Th1細胞激活

巨噬細胞，自然殺傷（NK）細胞和CD8 + T細胞，并負責細胞免疫。Th1細胞提供

保護對細胞內的細菌，真菌，原生動物和病毒和參與一些自身免疫反應。IL-4

由Th2細胞產生特別強的驅動B細胞產生IgE的分泌細胞。IgE嗜堿性粒細胞和肥大作用

細胞介導的免疫反應。Th2細胞介導的防外寄生蟲也可能導致有害的過敏

反應性發展。通過對IL-17A和其他因子的分泌，招募和激活中性粒細胞和Th17細胞

調解對胞外細菌和真菌的免疫反應。Th17細胞也參與自身免疫反應。在

除了這些類型的T輔助細胞，

應注意，BD golgistop™蛋白轉運抑制劑的出現可能清楚的顏色范圍

（無色到淡黃色）。

Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Irritating to eyes and skin. Do not breathe vapor. In case of contact with eyes, rinse immediay with plenty of water and seek medical
advice. Wear suitable protective clothing.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were
removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

A. Stimulation of the Cells
Various in vitro methods have been reported for polarization or stimulation of T helper cells subsets of which PMA (Phorbol ester) plus
Ionomycin (Calcium Ionophore) has been particularly useful for quickly inducing and characterizing polyclonal cytokine-producing cells. For this
kit we recommend the stimulation of normal PBMCs at a concentration of 1-10 million cells per ml in media for 5 hours with PMA/Ionomycin (at
50 ng/ml and 1 μg/ml respectively) in the presence of BD GolgiStop™ Protein Transport Inhibitor (provided in the kit or Cat #554724). Add 4 μl
of BD GolgiStop™ for every 6 ml of cell culture and mix thoroughly. It is recommended that BD GolgiStop™ not be kept in cell culture for
longer than 12 hours.
Note: Kinetic studies need to be performed to determine the optimal incubation time for each experimental system. Depending on the donor, frequencies of cytokine
producing cells derived from activation of PBMC can vary widely for a specific cytokine. In particular, the number of IL-17 producing cells can be very low or even
negligible on PMA/Ionomycin stimulated PBMC. In these cases, Th17 polarization cultures should be considered. For specifics on polarization of Th17 cells please refer
to the references.
560751

560741 HU IFN GMA NHP PE-CY7 MAB 50TST 4S.B3
560742 HU IFN GMA NHP PERCP-CY5.5 50TST 4S.B3
560743 HU HLA-DR ALEXA 700 MAB 50TST G46-6
560744 Hu HLA-DR NHP APC MAB 50TST G46-6
560746 10X PERM BUF IV 50ML
560747 HU CSM LYOPLATE SCREENING PANEL
560748 HU CD195 NHP APC MAB 50TST 3A9
560749 HU SOX1 PURE MAB 0.1MG N23-844
560750 HU T CELL ACT KIT 50TST
560751 HU TH1/2/17 PHNOTYP KIT 50TST
560752 HU TH1/17 PHNOTYP KIT 50TST
560753 HU REG TCELL SORT KIT
560754 Mouse CD335/Nkp 46 purified 29A1.4 0.5MG
560755 MS CD335 (NKP46) ALEXA 647 0.1MG 29A1.4
560756 MS CD335(NKP46)FITC MAB 0.1MG 29A1.4
560757 MS CD335 (NKP46) PE MAB 0.1MG 29A1.4
560758 MS TH1/2/17 PHNOTYP KIT 50TST
560759 MS TH1/17 PHNOTYP KIT 50TST
560761 HU CD4/CD8 T-CELL AB CKTL 50TST
560762 HU TH17/TREG PHNOTYP KIT 50TST
560763 MS CD335 (NKP46) V450 MAB 0.1MG 29A1.4
560764 MS CD335 (NKP46) V450 MAB 0.025MG 29A1.4
560765 HU CD197 (CCR7) PE MAB 100TST 150503
560766 MS CD197 (CCR7) ALEXA 647 MAB 0.1MG 4B12
560767 MS TH17/TREG PHNOTYP KIT 50TST
560768 HU CD4 HRZN V500 MAB 100TST RPA-T4
560769 HU CD4 HRZN V500 MAB 25TST RPA-T4
560770 NHP CD3 HRZN V500 MAB 100TST SP34-2
560771 MS CD3E HRZN V500 MAB 0.1MG 500A2
560772 NHP CD3 HRZN V500 MAB 25TST SP34-2
560773 MS CD3E HRZN V500 MAB 0.025MG 500A2
560774 HU CD8 HRZN V500 MAB 100TST RPA-T8
560775 HU CD8 HRZN V500 MAB 25TST RPA-T8
560776 MS CD8A HRZN V500 MAB 0.1MG 53-6.7
560777 HU CD45 HRZN V500 MAB 100TST HI30
560778 MS CD8A HRZN V500 MAB 0.025MG 53-6.7
560779 HU CD45 HRZN V500 MAB 25TST HI30
560780 MS CD44 HRZN V500 MAB 0.1MG IM7
560781 MS CD44 HRZN V500 MAB 0.025MG IM7
560782 MS CD4 HRZN V500 MAB 0.1MG RM4-5
560783 MS CD4 HRZN V500 MAB 0.025MG RM4-5
560784 RAT IGG2B KPA ITCL HRZN V500 0.1MG A95-1
560785 HAM IGG2 KPA ITCL HRZN V500 0.1MG B81-3
560786 RAT IGG2A KPA ITCL HRZN V500 .1MG R35-95
560787 MS IGG1 ITCL HRZN V500 MAB 0.1MG X40
560788 CRKL (PY207) PE MAB 50TST K30-391.50.80
560789 CRKL PY207 ALEXA 488 50TST K30-391.50.80
560790 CRKL PY207 ALEXA 647 50TST K30-391.50.80
560791 HU NANOG ALEXA 488 MAB 50TST N31-355
560792 HU GAPDH CBA FLEX SET 100TST A9
560793 HU TRA-1-81 ALEXA 647 FC 100TST TRA-1-81
560794 OCT-3/4 PERCP-CY5.5 50TST 40
560795 HU CD279 (PD-1) PE MAB 100TST EH12.1
560796 SSEA-4 ALEXA 647 FC 100TST MC813-70
560797 SAV V450 0.1MG
560798 2 CLR ITCL CKTL CD4 PERCPCY5.5 20TST
560799 HU IL-17A PERCP-CY5.5 MAB 100TST N49-653
560800 MS CD335 PERCP-CY5.5 MAB 0.1MG 29A1.4
560801 MS CD3E HRZN V450 MAB 0.1MG 500A2
560802 3 CLR ITCL CKTL CD4 PERCPCY5.5 20TST
560803 MS IGG3 KPA ITCL ALEXA 647 0.1MG J606
560804 MS CD3E HRZN V450 MAB 0.025MG 500A2
560805 MS CD197 (CCR7) V450 MAB 0.1MG 4B12
560806 MS IGM KPA ITCL ALEXA 647 0.1MG G155-228
560807 HU IL-9 PE MAB 100TST MH9A3
560808 BRDU PERCP MAB 50TST 3D4
560809 BRDU PERCP-CY5.5 MAB 50TST 3D4
560810 BRDU HRZN V450 MAB 0.05MG 3D4
560811 HU CD4 HRZN V450 MAB 50TST L200
560812 MS CD197 (CCR7) PERCP-CY5.5 0.1MG 4B12
560813 HU IL-9 ALEXA 647 MAB 100TST MH9A3
560814 HU IL-9 PE MAB 25TST MH9A3
560815 HU IL-9 ALEXA 647 MAB 25TST MH9A3
560816 HU CD197 (CCR7) ALEXA 647 100TST 150503
560817 I KPA B ALP ALEXA 647 MAB 50TST 25
560818 I KPA B ALP PE MAB 50TST 25
560819 HU CD105 PERCP-CY5.5 MAB 100TST 266
560820 MS IL-17A ALEXA 700 0.05MG TC11-18H10
560821 MS IL-17A APC-CY7 0.05MG TC11-18H10
560822 HU CD127 PE-CY7 50TST HIL-7R-M21
560823 HU CD127 Horizon V450 HIL-7R-M21 50Tst
560826 HU CD123 PE-CY7 MAB 50TST 7G3
560827 HU CD15 PE-CY7 MAB 50TST HI98
560828 HU CD15 PERCP-CY5.5 MAB 50TST HI98
560829 MS CD16/CD32 PE-CY7 MAB 0.05MG 2.4G2
560831 HU CD183 PE-CY7 MAB 50TST 1C6/CXCR3
560832 HU CD183 PERCP-CY5.5 50TST 1C6/CXCR3
560834 HU CD271 PERCP-CY5.5 MAB 50TST C40-1457
560835 HU CD3 PERCP-CY5.5 MAB 50TST UCHT1
560836 HU CD4 NHP ALEXA 700 MAB 50TST L200
560837 HU CD4 NHP APC-H7 MAB 50TST L200
560838 HU CD279 (PD-1) ALEXA 647 100TST EH12.1
560839 HU CD105 PE MAB 100TST 266
560840 HU CD49D FITC MAB 100TST 9F10
560842 HU CD56 PERCP-CY5.5 MAB 50TST B159
560844 HU CD57 PE MAB 100TST NK-1
560845 HU CD57 APC MAB 50TST NK-1
560846 HU CD146 FITC MAB 100TST P1H12
560847 HU CD73 APC MAB 100TST AD2
560850 HU TRA-1-60 AG ALEXA 647 100TST TRA-1-60
560851 HU MSI1 PURE MAB 0.1MG N14-47
560852 HU FOXP3 PE MAB 100TST 236A/E7
560853 HU CD20 NHP APC-H7 MAB 50TST 2H7
560854 HU NANOG CBA FLEX SET 100TST A8
560855 HU OCT-4 CBA FLEX SET 100TST A6
560856 MS IGM KPA ITCL PE-CY7 0.1MG G155-228
560857 MS IGM KPA ITCL PERCP-CY5.5 0.1MG G155-228
560858 AKT (PS473) HRZN V450 MAB 50TST M89-61
560861 MS IGM KPA ITCL HRZN V450 0.1MG G155-228
560862 HU REX-1 CBA FLEX SET 100TST C4
560863 HU CD197 (CCR7) HRZN V450 120TST 150503
560864 HU CD197 (CCR7) HRZN V450 30TST 150503
560867 HU IL-2 HRZN V450 MAB 50TST 5344.111
560868 MS IGD APC MAB 0.05MG 11-26C.2A
560869 MS IGD HRZN V450 MAB 0.05MG 11-26C.2A
560871 HU VEGFR-2/KDR ALEXA 647 25TST 89106
560872 HU VEGFR-2/KDR PE MAB 25TST 89106
560873 HU NANOG PE MAB 25TST N31-355
560874 HU CD144 FITC MAB 25TST 55-7H1
560875 MS CD184 HRZN V450 MAB 0.05MG 2B11/CXCR4
560876 HU TRA-1-60 AG FITC MAB 25TST TRA-1-60
560877 HU CD271 ALEXA 647 MAB 25TST C40-1457
560879 SSEA-3 PE MAB 25TST MC631
560881 SSEA-3 FITC MAB 25TST MC631
560882 MS IGG2A ITCL HRZN V450 0.1MG MOPC-173
560883 HU TRA-1-81 AG FITC MAB 25TST TRA-1-81
560884 HU TRA-1-60 AG PE MAB 25TST TRA-1-60
560885 HU TRA-1-81 AG PE MAB 25TST TRA-1-81
560886 SSEA-1 PE MAB 25TST MC480
560887 HU FOXP3 ALEXA 488 MAB 25TST 259D/C7
560889 HU FOXP3 ALEXA 647 MAB 25TST 259D/C7
560890 HU CD44 APC MAB 25TST G44-26
560891 RAT IGG2C KPA ITCL ALEXA 647 0.1MG A23-1
560892 RAT IGM KPA ITCL ALEXA 647 0.1MG R4-22
560893 RAT IGG2C KPA ITCL FITC MAB 0.1MG A23-1
560894 MS IGG2A ITCL ALEXA 700 0.1MG MOPC-173
560895 HU CD11C APC MAB 25TST B-LY6
560896 HU HLA-DR APC MAB 25TST G46-6
560897 MS IGG2A KPA ITCL APC-H7 0.1MG G155-178
560899 HU CD45RO APC MAB 25TST UCHL1
560900 HU CD20 APC MAB 25TST 2H7
560901 ACT CASPASE-3 FITC MAB 25TST C92-605
560902 HU IL-2 PE MAB 25TST MQ1-17H12
560903 HU CD166 PE MAB 25TST 3A6
560904 HU CD123 PERCP-CY5.5 MAB 25TST 7G3
560905 HU CD127 ALEXA 647 25TST HIL-7R-M21
560906 MS IGG2A KPA ITCL PE-CY7 0.1MG MOPC-173
560907 HU CD20 PERCP-CY5.5 25TST H1 (FB1)
560908 HU CD279 PE MAB 25TST MIH4
560909 HU CD4 PE-CY7 MAB 25TST SK3
560910 HU CD3 PE-CY7 MAB 25TST SK7
560911 HU CD19 PE-CY7 MAB 25TST SJ25C1
560912 HU CD69 APC-CY7 MAB 25TST FN50
560913 HU CD195 APC-CY7 MAB 25TST 2D7/CCR5
560914 HU CD11B/MAC-1 APC-CY7 MAB 25TST ICRF44
560915 HU CD45 PE-CY7 MAB 25TST HI30
560916 HU CD56 PE-CY7 MAB 25TST B159
560917 HU CD8 PE-CY7 MAB 25TST RPA-T8
560918 HU CD16 PE-CY7 MAB 25TST 3G8
560919 HU CD14 PE-CY7 MAB 25TST M5E2
560920 HU CD25 PE-CY7 MAB 25TST M-A251
560921 HU (CD197) CCR7 ALEXA 647 25TST 3D12
560922 HU (CD197) CCR7 PE-CY7 MAB 25TST 3D12
560923 HU TNF PE-CY7 MAB 25TST MAB11
560924 HU IFN-GMA PE-CY7 MAB 25TST B27
560925 HU CD80 PE MAB 25TST L307.4
560926 HU CD80 FITC MAB 25TST L307.4
560927 HU CD271 PE MAB 25TST C40-1457
560928 HU CD183 PE MAB 25TST 1C6/CXCR3
560929 HU CD83 FITC MAB 25TST HB15E
560930 ANNEXIN V RECOM PE 25TST
560931 ANNEXIN V RECOM FITC 25TST
560932 HU CD195 PE MAB 25TST 3A9
560933 HU CD163 PE MAB 25TST GHI/61
560934 HU CD221 PE MAB 25TST 1H7
560935 HU CD195 PE MAB 25TST 2D7/CCR5
560936 HU CD184 APC MAB 25TST 12G5
560937 HU CD184 PE-CY5 MAB 25TST 12G5
560938 HU CD152 APC MAB 25TST BNI3
560939 HU CD152 PE MAB 25TST BNI3
560940 HU CD34 APC MAB 25TST 581
560941 HU CD34 PE MAB 25TST 581
560942 HU CD34 FITC MAB 25TST 581
560943 HU HLA-DR PE MAB 25TST G46-6
560944 HU HLA-DR FITC MAB 25TST G46-6
560945 HU CD1A FITC MAB 25TST HI149
560946 HU CD107B FITC MAB 25TST H4B4
560947 HU CD107A PE-CY5 MAB 25TST H4A3
560948 HU CD107A PE MAB 25TST H4A3
560949 HU CD107A FITC MAB 25TST H4A3
560950 HU KPA FITC MAB 25TST G20-193