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DAPI Dilactate，MW 457.48
DAPI Dilactate，MW 457.48
Overview
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Ex/Em (nm) 358/461
MW 457.48
CAS # N/A
Solvent Water
Storage F/D/L
Category Nucleic Acid Detection
DNA Detection
Related Labeling Cells
Fluorescence Imaging
Biochemical Assays
DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures. DAPI dilactate has improved water solubility compared to DAPI HCl salt.
西安瑞禧生物科技有限公司是的熒光染料，我們提供的熒光染料的吸收波長和發射波長從300nm-810nm不等，我們可以再不同的熒光染料上面做的活性基團，比如NHS、amine、COOH、azide、alkyne、maleimide等等。關于熒光染料我們可以提供FITC、Rhodamine、Bodipy系列染料、Coumairn 染料、Dansyl染料、FAM/5-TAMRA、NBD、CY系列、ICG及IR系列、ATTO系列染料、AMCA、Perylene、碘化物、DIR/DIL/DIO/DID/DAPI膜染料等等。我公司特別在近紅外熒光染料Near infrared dyes方面有的產品線。
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