国产一卡2卡三卡4卡麻豆_了解最新日韩草逼视频_h片在线播放一区_国产激情影视在线_好了av四色综合无码久久_欧美黑白双插OOR720P_日本精品中文字幕在线_秋霞午夜手机影院_亚洲国产一区二区3da毛片_欧美杂交深喉video中文字幕

【文獻標題】Snail/HDAC1/2 mediate skeletal growth retardation in fetuses caused by prenatal nicotine exposure

【作者】Yu Deng , Hui Gao , Hui Wang , et.al

【作者單位】武漢大學（Wuhan University）

【文獻中引用產品】

試劑藏紅-O

試劑固綠FCF

【關鍵詞】Nicotine ,Snail ,Growth plate chondrodysplasia ,Histone acetylation

【DOI】doi.org/10.1016/j.tox.2021.152847

【影響因子(IF)】7.4

【出版期刊】《Toxicology》

【產品原文引用】

2.1. Chemicals and reagents 

Nicotine (CAS No. 54-11-5) and Isoflurane were purchased from Baxter Healthcare (Deerfield, IL, USA). Safranin-O (CAS no. 477-73-6) and fast green (CAS no. 2353-45-9) were obtained from Hengyuan Biotech (Shanghai, China). RIPA lysis buffer and BCA Protein Assay kit were purchased from Beyotime Institute of Biotechnology (Haimen, China). TRIzol reagent kits were obtained from Omega Bio-Tek (Doraville, GA, USA). Reverse transcription and quantitative PCR (qPCR) kits were purchased from TaKaRa Biotechnology Oligonucleotide primers for analyzed genes were synthesized by Sangon Biotech (Shanghai, China). The nucleus and cytoplasm extraction kit was purchased from Applygen Technologies Inc (Beijing, China). Pyruvate was purchased from Gibco (St. Louis, MO, USA). H3K9, H3K14, and IgG antibodies were obtained from Abcam (Cambridge, UK). DNA purification kit was purchased from Tiangen (Beijing, China). DMEM/F12 (1:1) and fetal bovine serum were supplied by Gibco. Polyclonal antibodies for Col2A1, ACAN, HDAC1, HDAC2, and Snail were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). si-Snail was purchased from Gene Pharma (Shanghai, China). Lipofectamine 2000 Reagent was obtained from Invitrogen (CA, USA). Penicillin was purchased from Harbin pharmaceutical Co. Ltd (Harbin, China). The remaining chemicals and 

reagents used were of analytical grade. 

完整版PDF文獻請咨詢在線客服或者聯系我司業務員！

更多公司福利請關注“恒遠生物"微信公眾號！