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產品名稱:人胰島素樣生長因子結合蛋白4(IGFBP-4 )
英文名稱:Human insulin-like growth factors binding protein 4,IGFBP-4 ELISA KIT
產品規格:96人份/48人份
主要組成成分:試劑盒
性狀:液體
適應癥:科研
貯藏:2-8℃
人胰島素樣生長因子結合蛋白4(IGFBP-4 )計算
以標準物的濃度為橫坐標,OD值為縱坐標,在坐標紙上繪出標準曲線,根據樣品的OD值由標準曲線查出相應的濃度;再乘以稀釋倍數;或用標準物的濃度與OD值計算出標準曲線的直線回歸方程式,將樣品的OD值代入方程式,計算出樣品濃度,再乘以稀釋倍數,即為樣品的實際濃度。
Steps description(英文)
Standard, Sample diluent |
Add Standard, Sample diluent, incubate for 30 min at |
Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at |
Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at |
Add Stopp Solution |
Read absorbance at 450nm within 15 min |
calculate |
Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
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