目錄:北京索萊寶科技有限公司>>細胞相關(guān)產(chǎn)品>>細胞培養(yǎng)試劑>> 31600DMEM（低糖，含雙抗） 細胞培養(yǎng)
| 供貨周期 | 現(xiàn)貨 | 規(guī)格 | 500ml |
|---|---|---|---|
| 貨號 | 31600 | 應用領(lǐng)域 | 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),石油 |
| 主要用途 | Cell culture tested, sterile reagent. w |
DMEM(低糖，含雙抗) 細胞培養(yǎng)
DMEM是一種含各種氨基酸和葡萄糖的培養(yǎng)基，高糖型有利于細胞停泊于一個位置生長，適于生長較快、附著較困難腫瘤細胞等。該類培養(yǎng)基廣泛應用于疫苗生產(chǎn)和各種初代病毒宿主細胞的細胞培養(yǎng)及單一細胞培養(yǎng);如A9、3T6、BALB/3T3、COS-1、COS-3、COS-7、L6、WEHI-3b等細胞系的培養(yǎng)。
DMEM培養(yǎng)基的配制方法：
1 制備新鮮三蒸水或Millipore超純水。
2 稱取所需量的干粉培養(yǎng)基，加入約終體積一半的三蒸水中;若配制一個包裝的培養(yǎng)液，在將整個包裝的干粉倒入三蒸水后，需用水洗包裝袋內(nèi)面2次，倒入培養(yǎng)液中，以保證所有干粉都溶解成培養(yǎng)液。磁力攪拌或人工攪拌使之*溶解。
3 根據(jù)包裝袋上的要求補加所需量的碳酸氫鈉;根據(jù)實驗需要，添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物質(zhì)。
4 加水定容到終體積。
5 必要時用1 mol/L 鹽酸和1 mol/L 氫氧化鈉調(diào)節(jié)pH。
6 用無菌0.22um濾膜過濾除菌，分裝于無菌血清瓶中，4℃冰箱保存。
配制好的培養(yǎng)液用前加入100U/mL青霉素和100U/mL鏈霉素，并根據(jù)需要加入血清(5%-20%)。
DMEM培養(yǎng)基的特點：
（1）氨基酸含量為依格爾培養(yǎng)基的2倍，且含有非必需氨基酸，如甘氨酸等；
（2）維生素含量為依格爾培養(yǎng)基的4倍；
（3）含有糖酵解途徑中的重要物質(zhì)——丙酮酸；
（4）含有微量的鐵離子。
DMEM培養(yǎng)基應用廣泛。基于DMEM培養(yǎng)基中氨基酸、微生物含量豐富，并具有糖酵解中的丙酮酸和微量Fe離子，因此在細胞培養(yǎng)、疫苗生產(chǎn)等方面的應用為常見。
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DMEM(低糖，含雙抗) 細胞培養(yǎng)
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