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北京西美杰科技有限公司
主營產品： 生物學檢測試劑盒,化學試劑,蛋白質氧化損傷,抗原抗體 |
15
聯系電話
18210960361
公司信息
- 聯系人：
- 西美杰
- 電話：
- 400-050-4006
- 手機：
- 18210960361
- 售后電話：
- 010-88597838
- 地址：
- 北京市海淀區金源時代商務中心B座10F
- 郵編：
- 100097
- 網址：
- www.xmjsci.com
[供應]A5260,0050-DNA Blood Isolation Spin-Kit（血液DNA提取試劑盒）
- 貨物所在地：
- 北京北京市
- 產地：
- 德國
- 更新時間：
- 2024-09-08 21:00:06
- 有效期：
- 2024年9月8日--2025年3月8日
- 已獲點擊：
- 290
【簡單介紹】DNA Blood Isolation Spin-Kit，A5260,0050，50 Isol
【詳細說明】
| HS-No. | 38220000 | |||
|---|---|---|---|---|
| Storage | RT | |||
| except for Proteinase K - Solution: +4°C | ||||
| • Kit for the DNA isolation from blood cells with spin minicolumns | ||||
| • Application of the DNA in PCR, cloning and sequencing | ||||
| • Maximum sample volume 100 μl | ||||
| • Binding capacity of the column 10 μg DNA | ||||
| • sufficient for 50 and 250 assays, respectively | ||||
| • Isolation of fragments in the range of 20 kb | ||||
The DNA Blood Isolation Spin-Kit is based on the ability of DNA to adsorb to silica surfaces in the presence of high concentrations of chaotropic salts. Blood cells are Iysed with the supplied lysis buffer, which contains chaotropic salts and a non-ionic detergent （TL buffer）. Cell lysis is additionally supported by the high-activity protease Proteinase K. In the next step, the whole sample is loaded onto a minicolumn with a special silica resin. The DNA binds to the silica resin, while contaminants （fragmented proteins, salts, etc.） pass freely through the column. Residual contaminations are removed by a washing step. Pure DNA is eluted with a low salt buffer or water and can be used in the following experiments without additional purification procedures （e.g. precipitation）.
