北京西美杰科技有限公司
主營產品: 生物學檢測試劑盒,化學試劑,蛋白質氧化損傷,抗原抗體 |
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18210960361
公司信息
- 聯系人:
- 西美杰
- 電話:
- 400-050-4006
- 手機:
- 18210960361
- 售后電話:
- 010-88597838
- 地址:
- 北京市海淀區金源時代商務中心B座10F
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- 100097
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[供應]A5253,0025-DNA Swab Isolation Spin-Kit(咽試子DNA提取試劑盒)
- 貨物所在地:
- 北京北京市
- 產地:
- 德國
- 更新時間:
- 2024-09-08 21:00:06
- 有效期:
- 2024年9月8日--2025年3月8日
- 已獲點擊:
- 598
【簡單介紹】DNA Swab Isolation Spin-Kit,A5253,0025,25 Isol
【詳細說明】
HS-No. | 38220000 | |||
---|---|---|---|---|
Storage | RT | |||
except for Proteinase K - Solution: +4°C | ||||
• Kit for the DNA isolation from swabs with spin minicolumns | ||||
• Advantage: approx. preparation time 30 minutes | ||||
• Binding capacity of the column 10 μg DNA | ||||
• sufficient for 25 and 100 isolations, respectively |
This kit is based on the property of DNA to adsorb to silica surfaces in the presence of high concentrations of chaotropic salts. The lysis of cells from swabs is performed in the lysis solution containing chaotropic salts and nonionic detergents. The lysis process is supported by auxiliary, high affinity protease (Proteinase K). Such reagents assure lysis and complete protein degradation. Subsequently the Iysate is loaded onto the spin minicolumn with dedicated silica membrane. The DNA binds to the silica membrane, while contaminants (fragmented proteins, salts. etc.) pass through the column freely. Residual contaminants are moved away by a wash step. Pure DNA is eluted with a low salt buffer or water and can be used in following experiments without further purification procedures (e.g. precipitation).