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北京西美杰科技有限公司
主營產(chǎn)品： 生物學(xué)檢測試劑盒,化學(xué)試劑,蛋白質(zhì)氧化損傷,抗原抗體 |
聯(lián)系電話
18210960361
公司信息
- 聯(lián)系人：
- 西美杰
- 電話：
- 400-050-4006
- 手機：
- 18210960361
- 售后電話：
- 010-88597838
- 地址：
- 北京市海淀區(qū)金源時代商務(wù)中心B座10F
- 郵編：
- 100097
- 網(wǎng)址：
- www.xmjsci.com
- 貨物所在地：
- 北京北京市
- 產(chǎn)地：
- 美國
- 更新時間：
- 2024-09-30 21:00:07
- 有效期：
- 2024年9月30日--2025年3月30日
- 已獲點擊：
- 970
PureExo® Exosome Isolation Kit (for serum or plasma)
Why our exosome so pure?
Answer: By targeted filtration, our filter of specific binding treatment removes protein contaminant to isolate exosome at 95% high purity.
Name
PureExo® Exosome Isolation Kit (for serum or plasma)
Cat. #
| P101S (2 reactions) contact Fisher P101(10 reactions) order from Fisher (Fisher Cat.# NC0874905) P101L(40 reactions) contact Fisher P101H (P101 Sample Buffer, 20 ml) We also offer purified exosome: P140 (Purified Normal Human Exosome, from serum) P141 (Purified Cancer / Disease Exosome, from patient serum) |
Application
| Isolate pure exosome from serum and plasma. This kit yields much higher purity than exosome precipitation method. This product is for research use only. |
Yield
| Each reaction can process 100 ~ 500 µL serum/ plasma. Each reaction can yield pure exosome suspended in 100 ~ 200 µL PBS (from which 300 ~ 400 µg exosomal protein or 200 ~ 300 ng exosomal RNA can be extracted using our Exosomal RNA and Protein Extraction Kit, Cat.#: P200). |
Description
P101 kit is for exosome purification / isolation at high yield and purity from serum or plasma.
Use as little as 100 µL serum to achieve high yield of exosomes for any downstream applications: EM study, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins. |
Shipping / Storage
| Ship and store at room temperature. Upon receiving keep all bottles upright, in cool and dark place. |
Shelf Life
| 12 months |
Updated VersionManual (protocol)
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Serum and plasma preparation
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Citation
Citation 1: Chen L, Chen R, Kemper S, Charrier A, Brigstock DR. Suppression of fibrogenic signaling in hepatic slate cells by Twist1-dependent microRNA-214 expression: Role of exosomes in horizontal transfer of Twist1. Am J Physiol Gastrointest Liver Physiol. 2015 Sep 15;309(6):G491-9. doi: 10.1152/ajpgi.00140.2015. Epub 2015 Jul 30.
This paper is awarded *0 best original research papers published by the American Physiological Society (APS Select). Link (APSselect is a collection of the very best original research papers published by the American Physiological Society. Each month, the most outstanding recently published papers from our ten research journals are selected and made available through this multi-journal website.) Citation 2: Osteikoetxea X, Németh A, Sódar BW, Vukman KV, Buzás EI. Extracellular vesicles in cardiovascular diseases, are they Jedi or Sith?
Citation 3: William G. Whitford Exosomes: A Major Phenomenon Flying Under the Radarcellculturedish.com 2015/09
Citation 4: Chen L., Chen R., Brigstock D. MicroRNA profiling of circulating exosomes during experimental liver fibrosis. Hepatology. AASLD abstract, 2014 Oct. (Presidential Honored Distinct Abstract).
Citation 5: Li Chen and David R. Brigstock. Cellular or Exosomal microRNAs Associated with CCN Gene Expression in Liver Fibrosis. CCN Proteins. Volume 1489 of the series Methods in Molecular Biology pp 465-480.
Citation 6: Kayo Nakamura, Masatoshi Jinnin*, Miho Harada, Hideo Kudo, Wakana Nakayama, Kuniko Inoue, Aki Ogata, Ikko Kajihara, Satoshi Fukushima, Hironobu Ihn. Altered expression of CD63 and exosomes in scleroderma dermal fibroblasts. J Dermatol Sci.2016 Oct;84(1):30-39. doi: 10.1016/j.jdermsci.2016.06.013. Epub 2016 Jul 1.
Citation 7: Li Chen and David R. Brigstock. Integrins and heparan sulfate proteoglycans on hepatic slate cells (HSC) are novel receptors for HSC-derived exosomes. FEBS Lett. 2016 Dec;590(23):4263-4274. doi: 10.1002/1873-3468.12448. Epub 2016 Oct 23.
Link to all 101Bio citations |
FAQ
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Components
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Remarks
| Because the exosome yield of this exosome isolation kit is 10-fold higher than all other kits, you can start with as little as 100 µL serum. One PureExo® Column can be used only for one reaction. Do not process more than 500 µL serum/plasma per each reaction. Otherwise it may cause column clogging. |
Our kits optimize your exosome research result
