89802-- I-PER Insect Cell Protein Extraction Reagent
- 公司名稱 北京華夏遠洋科技有限公司
- 品牌 Thermofisher Scientific/賽默飛世爾
- 型號 89802--
- 產地 Thermo pierce
- 廠商性質 代理商
- 更新時間 2017/9/3 23:20:47
- 訪問次數 754
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I-PER Insect Cell Protein Extraction Reagent
Efficient extraction of recombinant proteins from insect cells. Cell lysates were prepared from infected Sf9 cells using Thermo Scientific I-PER Insect Protein Extraction Reagent. Normalized amounts of total, insoluble and soluble protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE） before Western blot analysis.
Affinity purification of 6xHis Cyclin B1 from insect cell extract. Baculovirus-infected Sf9 cells were harvested and lysed with Thermo Scientific I-PER Reagent. The extract was directly loaded onto a nickel-chelated agarose （IMAC） column and purified. Protein samples were separated by SDS-PAGE, and the gel was stained with GelCode Blue Stain Reagent （Part No. 24590）.
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Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price 89802
Sufficient For: 5 to 20 million cultured cells per 1mL of reagent250mL 
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