3D Cell Culture Chip 三維細胞培養芯片

材料

芯片采用生物相容性聚二甲基硅氧烷（PDMS）制成，具有透氣性，保證了CO2和O2的交換薄玻璃載體是一種具有高透明、低自熒光等優良光學性能的顯微鏡載玻片。這允許通過光學顯微鏡、熒光顯微鏡和共焦顯微鏡以時間和空間分辨率直接觀察生物樣品。

技術特點

邊溝中間溝

高度150微米150微米

寬度

3250微米

1125微米（狹窄部分）

3375微米（寬部分）

體積（建議注射）

20微升20微升

入口直徑1000微米1000微米

芯片表面沒有涂層。根據您的應用，并終改善細胞粘附性，您可以使用聚合物和/或蛋白質溶液來覆蓋通道表面。

這種微流控裝置還可以與細胞嵌入水凝膠（如I型膠原、IV型膠原、纖維蛋白、基質凝膠等）中，以重建可靠的生物微環境。

入口直徑為1毫米，可通過普通吸管輕松注入細胞懸液20微升是每個通道/腔室的推薦體積；這將允許在實際注射和*填充時有少量額外的微升。

此外，還可以使用直接安裝在入口的1/16“管子在動態流動條件下進行研究。

好處

使用方便

生理相關微環境

單細胞分辨率

直接可視化

時空分辨率

定性和定量評估

3D Cell Culture Chip 三維細胞培養芯片

Materials

The IC-Chip is made of biocompatible polydimethylsiloxane (PDMS) and is gas-permeable to ensure the CO2 and O2 exchange. The support in thin glass is a microscope slide with excellent optical properties such as high transparency and low autofluorescence. This allows a direct observation of your biological sample with temporal and spatial resolution by using optical, fluorescent and confocal microscopes.

Technical features

The IC-Chip has not coated surfaces. According to your application and to eventually improve cell adhesion, you can coat the channel surface by using your solutions of polymers and/or proteins. 

This microfluidic device can be used also with cells embedded in hydrogels such as Collagen I, Collagen IV, Fibrin, Matrigel, etc. to recreate a reliable biological microenvironment. 

The inlet diameter of 1 mm allows an easy injection of the cell suspension through common pipettes. 20 µL is the recommended volume for each channel/chamber; this will allow to have few extra µL for a practical injection and complete filling.

Additionally, it would be possible to perform studies in dynamic flow conditions by using 1/16” tubing directly fitted in the inlets. 

Benefits

• Easy-to-use
• Physiologically relevant microenvironment 
• Single cell resolution 
• Direct visualization
• Temporal and spatial resolution 
• Qualitative and quantitative assessments