柔軟的細胞培養板(皿),讓細胞不再睡在硬板床上
*,體內細胞所處周邊環境的軟硬度 (softness,stiffness)可謂千差萬別,可我們體外培養細胞時卻選擇了使用幾乎相同材質,硬邦邦的培養板(皿)。硬邦邦的培養板(皿)的硬度(E)達107kPa,而實際上,各組織細胞所處環境的硬度均低于100kPa,例如,E(小鼠胚胎干細胞)=0.6kPa,E(肝成纖維細胞)=1kPa,E(肝癌細胞)=1kPa,E(胚胎心肌細胞)=11kPa??梢?,給細胞一個類似母體的舒適環境,更能正確的反映細胞生物學特征。
彈性指數(E)是指材質的硬度(柔軟度),普通 培養板(皿) 的硬度要比身體組織硬105-109倍
Softwell 細胞培養板(皿)介紹
根據不同細胞類型設計生產了硬度各異的透明水凝膠,并將這些水凝膠鋪設在我們常用細胞培養板、皿等用材的表面,薄薄的水凝膠讓您感覺不到它的存在,但細胞卻可以明顯感受到它的柔軟、舒適,從而讓細胞宛如生長在母體的組織環境中。 例如可讓神經細胞如同培養在腦組織中 ,讓心肌細胞如同在肌肉組織中生長,或者如同任何細胞的同源器官環境,從而讓您的實驗數據更準確。
Softwell系列產品表面水凝膠的優點:
1. ?。?/strong>只有幾毫米,肉眼幾乎看不到
2. 與培養板(皿)緊密結合:對胰酶消化、洗滌細胞、傳代均無影響
3. 透明:*光學穿透性,可用于顯微鏡觀測
4. 2D系統:細胞生長在凝膠表面,便于細胞分離、分析
5. 安全:對細胞無任何毒性
6. 穩定:室溫下可保存6個月
Softwell系列產品優勢:
1. 適用于任何細胞,如神經細胞、胚胎干細胞、心肌細胞等
2. 2D細胞培養,卻能給細胞宛如母體的生長環境
3. 可自行包被EMC蛋白或選擇包被好的產品
Softwell系列產品種類很多,選擇方法如下:
1. 選擇不同功能的水凝膠,有三個系列
水凝膠功能 | ||
Easy coat 系列 | Collagen I 系列 | Non-Activated 系列 |
水凝膠已活化,可根據自身實驗需要自行包被EMC蛋白,如膠原蛋白、纖連蛋白、層粘連蛋白等 | 水凝膠上已包被好I型膠原蛋白(取自鼠尾或綿羊皮),為即拆即用型,使用便捷 | 水凝膠未活化,其表面結合活性極低。用于懸浮細胞培養或自行活化 |
2. 選擇不同硬度的水凝膠
彈性指數 (kPa) | ||
Soft (柔軟) | Intermediate (中度) | Stiff (硬) |
0.2 0.5 1 | 2 4 8 | 12 25 50 |
適用于培養脂肪、肝、肺、胚胎、脂肪、神經部位的細胞 | 適用于培養肌肉、心臟部位的細胞 | 適用于培養骨骼部位的細胞 |
3. 選擇不同樣式的培養器皿
Softwell®系列
聚苯乙烯材質的水凝膠培養板,規格有6孔、12孔、24孔、96孔
Softwell GTM 系列
黑色聚苯乙烯框架、硼硅玻璃孔徑的水凝膠培養板,規格有6孔、12孔、24孔、96孔、384孔
PetrisoftTM 系列
聚苯乙烯材質的水凝膠培養皿,規格有35mm、100mm、150mm
SoftviewTM系列
35mm培養皿,里面放置覆蓋水凝膠的,高透度玻璃(直徑為10mm和20mm)
SoftslipTM 系列
內含水凝膠玻璃蓋玻片的培養板,可取出蓋玻片觀察,規格有6孔、12孔、24孔
SoftslideTM 系列
內含水凝膠的培養腔室玻片系統,規格為16孔板
產品目錄如下
Softwell® 系列:聚苯乙烯材質的水凝膠培養板
品名 | 貨號 | 水凝膠功能 | 硬度(kpa) |
6孔板 | SW6-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-EC-HTS | Easy Coat | HTS |
6孔板 | SW6-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-COL-HTS | Collagen I | HTS |
6孔板 | SW6-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96-NA-HTS | Non-Activated | HTS |
Softwell GTM 系列:黑色聚苯乙烯框架、硼硅玻璃孔徑的水凝膠培養板
品名 | 貨號 | 水凝膠功能 | 硬度(kpa) |
6孔板 | SW6G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-EC-HTS | Easy Coat | HTS |
384孔板 | SW384G-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
384孔板 | SW384G-EC-HTS | Easy Coat | HTS |
6孔板 | SW6 G-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12G-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24G-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-COL-HTS | Collagen I | HTS |
384 孔板 | SW384G-COL | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
384孔板 | SW384G-COL-HTS | Easy Coat | HTS |
6孔板 | SW6G -NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板 | SW12G-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板 | SW24G-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
96孔板 | SW96G-NA-HTS | Non-Activated | HTS |
384 孔板 | SW384G-NA | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
384孔板 | SW384G-NA-HTS | Easy Coat | HTS |
PetrisoftTM系列:聚苯乙烯材質的水凝膠培養皿
品名 | 貨號 | 水凝膠功能 | 硬度(kpa) |
35mm培養皿 | PS35-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
100mm培養皿 | PS100-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
150mm培養皿 | PS150-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養皿 | PS35-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
100mm 培養皿 | PS100-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
150mm 培養皿 | PS150-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養皿 | PS35-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
100mm 培養皿 | PS100-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
150mm 培養皿 | PS150-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
SoftviewTM系列: 35mm培養皿,里面放置覆蓋水凝膠的高透度玻璃
品名 | 貨號 | 水凝膠功能 | 硬度(kpa) |
35mm 培養皿/10mm 玻片 | SV3510-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養皿/10mm玻片 | SV3510-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養皿/10mm玻片 | SV3510-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養皿/20mm玻片 | SV3520-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養皿/20mm玻片 | SV3520-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
35mm 培養皿/20mm玻片 | SV3520-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
SoftslipTM系列:內含水凝膠玻璃蓋玻片的培養板
品名 | 貨號 | 水凝膠功能 | 硬度(kpa) |
6孔板/ 30 mm 蓋玻片 | SS6-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板/ 18 mm蓋玻片 | SS12-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板/ 12 mm蓋玻片 | SS24-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
6孔板/ 30 mm蓋玻片 | SS6-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板/ 18 mm蓋玻片 | SS12-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板/ 12 mm蓋玻片 | SS24-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
6孔板/ 30 mm蓋玻片 | SS6-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
12孔板/ 18 mm蓋玻片 | SS12-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
24孔板/ 12 mm蓋玻片 | SS24-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
SoftslideTM系列:內含水凝膠的培養腔室玻片系統
品名 | 貨號 | 水凝膠功能 | 硬度(kpa) |
16 孔培養腔室 | SSL16-EC | Easy Coat | 0.2,0.5,1,2,4,8,12,25,50,screen |
16 孔培養腔室 | SSL16-COL | Collagen I | 0.2,0.5,1,2,4,8,12,25,50,screen |
16 孔培養腔室 | SSL16-NA | Non-Activated | 0.2,0.5,1,2,4,8,12,25,50,screen |
該廠商的其他產品
- PS150-NA-xx PK 彈性150mm培養皿Petrisoft-150mm dish-Non Activated-xx pK
- PS100-NA-xx PK 彈性100mm培養皿Petrisoft-100mm dish-Non Activated-xx pK
- PS35-NA-xx PK 彈性35mm培養皿Petrisoft-35mm dish-Non Activated-xx pKa
- PS150-COL-xx PK 彈性150mm培養皿Petrisoft-150mm dish-Collagen I-xx pKa
- PS100-COL-xx PK 彈性100mm培養皿Petrisoft-100mm dish-Collagen I-xx pKa
- PS35-COL-xx PK 彈性35mm培養皿Petrisoft-35mm dish-Collagen I-xx pKa
- PS150-EC-xx PK 彈性150mm培養皿Petrisoft-150mm dish-easy coat-xx pKa
- PS100-EC-xx PK 彈性100mm培養皿Petrisoft-100mm dish-easy coat-xx pKa