国产一卡2卡三卡4卡麻豆_了解最新日韩草逼视频_h片在线播放一区_国产激情影视在线_好了av四色综合无码久久_欧美黑白双插OOR720P_日本精品中文字幕在线_秋霞午夜手机影院_亚洲国产一区二区3da毛片_欧美杂交深喉video中文字幕

 

作者： Jing Hana, b, Xuebing Lia,

a CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 8 Zhongguancun Beiertiao, Beijing 100190, China

b Graduate University, Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing 100049, China

 

摘要：This paper describes the facile synthesis and bioevaluations of a novel conjugated polymer having tetrasaccharide SLeX branch and chitosan backbone (SLeX–chitosan conjugate). The synthesis was achieved by a convenient chemoenzymatic approach using an aldehyde-functionalized N-acetylglucosamine to branch it onto chitosan amino groups followed by three enzymatic reactions to further append galactose, sialic acid, and fucose residue to the branch. Surface plasmon resonance (SPR) study of SLeX–chitosan conjugate revealed a high affinity binding with E-selectin (Kd = 920 nM) and a potent inhibitory effect on the binding of E-selectin with SLeX–BSA (IC50 = 240 μM). By using biocompatible chitosan as the scaffold for presenting SLeX ligands, as well as the concise route tailored for the conjugate syntheses, the present study provides a practical method to explore safe and efficient anti-inflammatory agents.

 

關(guān)鍵詞：Chitosan; Sialyl Lewis X; Conjugated polymer; E-selectin; Anti-inflammatory agent