目錄:上海華雅思創生物科技有限公司>>細胞培養>>DC-CIK治療細胞治療>> Cygnus_Technologies F640 Pichia pastoris 2nd Generation
產品英文名稱：Pichia pastoris 2nd Generation HCP ELISA kit
產品中文名稱：畢赤酵母第二代 HCP 試劑盒
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Pichia pastoris 2nd Generation HCP ELISA kit 的應用
本試劑盒是用于確定在畢赤酵母中的重組表達的宿主細胞生產的產品的畢赤酵母蛋白污染的存在。Cygnus賣畢赤酵母HCP檢測的兩種ELISA試劑盒。原 盒F140，有一個非常廣泛的反應性的肽表位，此外，還有一個小群體的抗體識別的抗原表位的畢赤酵母的糖基化。在重組蛋白產品同時糖化，有交叉反應情況 下，可以導致在真正的hcp或不定的hcp濃度由于樣本稀釋線性估計潛在的缺乏。用F640試劑盒用的抗體是與F140試劑盒相同的多克隆抗體是除了已經 承受額外的親和吸附去除抗體的糖基化表位。該試劑盒是用于研究和制造使用，不用于人類或動物的診斷應用。
畢赤酵母第二代 HCP 試劑盒的英文描述
Our original F140 kit, has a very broad reactivity to peptide epitopes and in addition, also has a small population of antibodies that recognize Pichia glycosylation epitopes. In cases where the recombinant protein product is also glycosylated, there is potential for some cross reactivity that can result in either an over-estimation of true HCP or indeterminate HCP concentrations, due to the lack of sample dilutional linearity.
The antibody used in this kit is the same polyclonal antibody that is used in the F140 kit, except that it has been subjected to an additional affinity adsorption step to remove antibodies to glycosylation epitopes and is much more specific than the F140, Pichia pastoris HCP ELISA kit.
Pichia pastoris 2nd Generation HCP ELISA kit 的使用步驟規則
The Pichia pastoris assay is a two-site immunoenzymetric assay. Samples containing Pichia pastoris HCPs are reacted in microtiter strips coated with an affinity purified capture antibody. A second horseradish peroxidase (HRP) enzyme labeled anti-Pichia pastoris antibody is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of Pichia pastoris HCPs present.
畢赤酵母第二代 HCP 試劑盒的保存和穩定性
1 All reagents should be stored at 2℃ to 8C℃for stability until the expiration date printed.
2 Reconstituted wash solution is stable until the expiration date of the kit.
Pichia pastoris 2nd Generation HCP ELISA kit的使用注意事項
1 只用于研究或制造。
2 停止反應試劑是0.5N 硫酸。避免接觸眼睛，皮膚，和服裝。在用本試劑盒的試劑濃度，其他沒有被認為是有害的。
3 本試劑盒只能由合格的技術人員使用。
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