目錄:上海華雅思創(chuàng)生物科技有限公司>>蛋白因子>>干細(xì)胞蛋白因子>> 小鼠Wnt-3a蛋白抑制劑(WNT3A)信號通路(5ug,W3A-M-005,r&d )
小鼠Wnt-3a蛋白抑制劑(WNT3A)信號通路(5ug,W3A-M-005,r&d )簡述:
訂購:
:gnana@hystemcell.com
WNT-3a是WNT信號蛋白家族的一員,扮演一個在胚胎發(fā)育和成年組織的完整性關(guān)鍵的角色。WNT-3a是對于規(guī)范通過beta-catenin WNT信號通路。小鼠WNT-3a的預(yù)測大小是一個含有333個氨基酸殘基的單體的蛋白質(zhì)。由于糖基化,它的表觀分子量40間遷徙kDa縮條件下分別分析。StemRD的產(chǎn)品從小鼠細(xì)胞序列段表示。
干細(xì)胞生長分化因子與人間充質(zhì)干細(xì)胞是屬于研究干細(xì)胞科學(xué)zui常用的試劑與培養(yǎng)基,目前各大研發(fā)機(jī)構(gòu)都從本公司購買,兩者一起購買優(yōu)惠哦!
小鼠Wnt-3a蛋白抑制劑(WNT3A)信號通路(5ug,W3A-M-005,r&d )英文簡述:
WNT-3a is a member of the WNT family of signaling proteins that play a key role in embryonic development and the integrity of adult tissues. WNT-3a is a prototypic canonical WNT that signals through the beta-catenin pathway. The predicted size of mouse WNT-3a is a monomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of 40 kDa by SDS-PAGE analysis under non-reducing conditions. StemRD’s product is expressed from a mouse cell line over-expressing mouse WNT-3a. We express and purify WNT proteins by using a proprietary process that is distinctive from the published protocol. Our method results in WNT proteins that are purer and more active than market-leading products which have been associated with non-specific artifacts.
小鼠Wnt-3a作為上海華雅思創(chuàng)代理temRD公司的系列產(chǎn)品之一,其他相關(guān)系列產(chǎn)品還有:
小鼠Wnt-3a
小鼠Wnt-3a蛋白抑制劑(WNT3A)信號通路(25ug,W3A-M-025,r&d )
小鼠Wnt-3a蛋白抑制劑(WNT3A)信號通路(100ug,W3A-M-100,r&d )
產(chǎn)品英文名稱:StemRD W3A-M-100 WNT-3a,Mouse,100ug
產(chǎn)品中文名稱:StemRD W3A-M-100 小鼠WNT-3a 蛋白因子,100ug
StemRD W3A-M-100 WNT-3a,Mouse,100ug 產(chǎn)品簡介
wnt通路是細(xì)胞增殖分化的關(guān)鍵調(diào)控環(huán)節(jié), 在胚胎發(fā)育和腫瘤發(fā)生中起著重要作用。wnt途徑參與了基因表達(dá)調(diào)節(jié)、 細(xì)胞遷移粘附、細(xì)胞極化等過程,同時還與其它信號通路存在交叉協(xié)同。Wnt/β-catenin通路在進(jìn)化過程中高度保守,此通路的主要分子構(gòu)成及相關(guān)調(diào) 控機(jī)制已得到基本闡明。對神經(jīng)系統(tǒng)而言已有足夠證據(jù)顯示此通路參與了對神經(jīng)前體細(xì)胞增殖, 分化以及決定細(xì)胞命運(yùn)的調(diào)控。
Wnt-3a 干細(xì)胞生長分化因子是Wnt基因家族中重要成員,zui近的一些研究發(fā)現(xiàn), Wnt-3a 干細(xì)胞生長分化因子信號蛋白對神經(jīng)干細(xì)胞的定向分化的調(diào)控作用表現(xiàn)的十分明顯。小鼠WNT-3a 干細(xì)胞生長分化因子蛋白包含328個氨基酸。
Wnt蛋白是存在于多種生物體內(nèi)的一種細(xì)胞外配體,Wnt作為形態(tài)發(fā)生素通過激發(fā)細(xì)胞內(nèi)遠(yuǎn)離信號發(fā)送區(qū)域的濃度依賴反應(yīng)控制胚胎形態(tài)發(fā)育。Wnt通路調(diào)節(jié)動物 發(fā)育過程中的多個重要環(huán)節(jié),例如細(xì)胞增殖、細(xì)胞遷徙及細(xì)胞分化。Wnt蛋白通過自分泌或旁分泌作用與位于細(xì)胞膜上的受體相結(jié)合,激活細(xì)胞內(nèi)信號通路調(diào)節(jié)靶 基因的表達(dá),對細(xì)胞的增殖、分化、遷移、極性化和凋亡起到重要作用。
WNT 信號轉(zhuǎn)導(dǎo)通路因其啟動蛋白為Wnt蛋白而得名,在胚胎發(fā)育、細(xì)胞增殖及分化等方面發(fā)揮重要 作用,其異常活化與人類多種腫瘤的發(fā)生密切相關(guān)。研究顯示,Wnt信號至少存在經(jīng)典和非經(jīng)典兩條不同的信號通路,而Wnt-5a屬于非經(jīng)典Wnt信號通 路,既往研究Ror2具有Wnt受體結(jié)構(gòu)域樣結(jié)構(gòu)。有研究認(rèn)為Wnt-5a經(jīng)由Ror2介導(dǎo)的 信號通路發(fā)揮作用。
Human WNT-5a蛋白含有357個氨基酸殘基。
StemRD W3A-M-100 小鼠WNT-3a 蛋白因子,100ug 產(chǎn)品特性
? StemRD W3A-M-100 小鼠WNT-3a 蛋白因子,100ug 干細(xì)胞生長分化因子活性經(jīng)過NIH 3T3 細(xì)胞鑒定,純度高
? StemRD W3A-M-100 小鼠WNT-3a 蛋白因子,100ug干細(xì)胞生長分化因子生物活性在NIH 3T3 細(xì)胞經(jīng)過TCF報告基因?qū)嶒炶b定The EC50為 50–150 ng/ml
? StemRD W3A-M-100 小鼠WNT-3a 蛋白因子,100ug 干細(xì)胞生長分化因子產(chǎn)品經(jīng)過SDS-PAGE 和HPLC雙層鑒定純度大于90%
StemRD W3A-M-100 WNT-3a,Mouse,100ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.
StemRD W3A-M-100 WNT-3a,Mouse,100ug 產(chǎn)品英文描述
WNT-3a is a member of the WNT family of signaling proteins that play a key role in embryonic development and the integrity of adult tissues. StemRD W3A-M-100 WNT-3a,Mouse,100ug is a prototypic canonical WNT that signals through the β-catenin pathway. The predicted size of mouse StemRD W3A-M-100 WNT-3a,Mouse,100ug is a monomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of 40 kDa by SDS-PAGE analysis under nonreducing conditions. StemRD’s product W3A-M-100 WNT-3a,Mouse,100ug is expressed from a mouse cell line overexpressing mouse WNT-3a. Purification is performed with a proprietary process that is distinct from the published method.
? StemRD W3A-M-100 WNT-3a,Mouse,100ug Purity: Greater than 90% as determined by SDS-PAGE analysis
? StemRD W3A-M-100 WNT-3a,Mouse,100ug Biological Activity: The activity was determined by using a TCF reporter gene assay in NIH3T3 cells. The EC50 ranges from 50 - 150 ng/ml. Country of Origin USA
StemRD W3A-M-100 小鼠WNT-3a 蛋白干細(xì)胞生長分化因子系列產(chǎn)品訂購信息
StemRD W3A-M-005 WNT-3a,Mouse 5ug 優(yōu)惠價格請
StemRD W3A-M-025 WNT-3a,Mouse 25ug 優(yōu)惠價格請
StemRD W3A-M-100 WNT-3a,Mouse 100ug 優(yōu)惠價格請
StemRD W5A-M-005 WNT-5a,Mouse 5ug 優(yōu)惠價格請
StemRD W5A-M-025 WNT-5a,Mouse 25ug 優(yōu)惠價格請
StemRD W3A-M-100 WNT-5a,Mouse 100ug 優(yōu)惠價格請
For Research Use Only. Not for Use in Humans.
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