目錄:上海華雅思創生物科技有限公司>>培養基>>生長分化因子>> StemRD_WNT-5a, Human 5µg 生長分化因子
產品英文名稱: WNT-5a, Human
產品中文名稱:人重組WNT-5a蛋白
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WNT-5a, Human產品介紹:
Wnt信號轉導通路因其啟動蛋白為Wnt蛋白而得名,在胚胎發育、細胞增殖及分化等方面發揮重要 作用,其異常活化與人類多種腫瘤的發生密切相關。研究顯示,Wnt信號至少存在經典和非經典兩條不同的信號通路,而Wnt-5a屬于非經典Wnt信號通 路,既往研究Ror2具有Wnt受體結構域樣結構。有研究認為Wnt-5a經由Ror2介導的 信號通路發揮作用。
Human WNT-5a蛋白含有357個氨基酸殘基。
WNT-5a, Human 產品特性:
? 活性在共轉Frizzled- 4 和 LRP- 5基因的HEK293 細胞鑒定,純度高,無動物成分污染
? WNT-5a, Human活性在共轉Frizzled- 4 和 LRP- 5基因的HEK293 細胞鑒定
? WNT-5a, Human產品經過SDS-PAGE 和HPLC雙層鑒定純度大于85%
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WNT-5a, Human產品英文簡述:
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical" pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
? Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.