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供貨周期 | 兩周 | 應用領域 | 醫療衛生,制藥 |
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Product Description
Transforming Growth Factor-alpha (TGF-α) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines. TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells.Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.
Product Properties
Synonyms | Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF |
Accession | P01135 |
Gene ID | 7039 |
Source | E.coli-derived Human TGF-a protein, Vla40-Ala89 |
Molecular Weight | Approximately 5.8 kDa, on SDS-PAGE under reducing conditions. |
AA Sequence | V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA |
Tag | None |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | > 95% as determined by SDS-PAGE |
Biological Activity | Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is < 0.4 ng/mL. |
Endotoxin | < 1.0 EU per 1μg of the protein by the LAL method. |
Formulation | Lyophilized after extensive dialysis against PBS. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -80℃ for 1 year from date of receipt.
1week, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.For your safety and health, please wear lab coats and disposable gloves for operation.
2.For research use only!