廣州測迪生物科技有限公司
主營產品: 美國kernel試劑盒,德國拜發試劑盒,瑞士Prionics試劑盒,荷蘭賽迪試劑盒 |
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廣州測迪生物科技有限公司
主營產品: 美國kernel試劑盒,德國拜發試劑盒,瑞士Prionics試劑盒,荷蘭賽迪試劑盒 |
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更新時間:2018-05-05 13:20:44瀏覽次數:448
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美國Kernel減蛋綜合征抗體檢測試劑盒
雞產蛋下降綜合癥也稱雞減蛋綜合癥。本病是70年代后期發現的,是世界性的商品蛋雞和母雞產蛋下降的一種病毒性疾病。群發性產蛋下降、產蛋異常、蛋體畸形、蛋質低劣等癥狀狀是本病患雞的主要表現。盡管它只對產蛋雞致病,但其自然宿主是家鴨和野鴨。
Egg drop syndrome (EDS) is characterized by production of soft-shelled and shell-less eggs in apparently healthy birds. It has been recognized worldwide, except in the USA. It replicates well in chick kidney or chick-embryo liver cells and to a lesser degree in chick-embryo fibroblasts. The natural hosts for EDS virus are ducks and geese, and the disease has been described in Japanese quail ( Coturnix coturnix japonica ). Three types of disease are recognized in chickens. Classical EDS probably was due to contamination of a vaccine for Marek’s disease grown in duck-embryo fibroblasts and subsequent adaptation of the virus to chickens. Basic breeding stock was infected, and the virus was transmitted vertically through the egg. The virus often remained latent until the chick reached sexual maturity, when it was excreted in the eggs and droppings to infect susceptible contacts. Because the virus is vertically transmitted and is reactivated around peak egg production, there was an apparent breed and age susceptibility. However, all ages and breeds of chickens are susceptible, although the disease tends to be most severe in heavy broiler-breeders or brown egg producers. Arising from the classical form, endemic EDS has been reported in many areas and is usually seen in commercial egg producers. Flocks become infected at any stage in lay. Contaminated egg collection trays are one of the main forms of horizontal transmission, and outbreaks are often associated with a common egg-packing station.
INTENDED USE
ELI-EDS ELISA kit is used for quantitative detection of antibodies against Egg Drop Syndrome virus (EDS) in chicken serum and yolk.
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-EDS antibodies present in the serum will bind to the EDS on the microwell forming an EDS antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the EDS antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 - 410 nm with a 630-650 nm reference filter.
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