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ATCC 750 熱帶念珠菌使用說明
組成：凍干菌種瓶、菌種復溶液（用于復溶菌種及保存用）、使用說明書。
方法：建議用下列方法恢復培養冷凍干燥的菌種。
復蘇菌種前應準備適于該菌種生長的固體或液體培養基和相應的培養設備，所有操作均應在符合生物安全保護及無菌條件下進行。
－、疫苗瓶開封
1．拉開易撕鋁蓋，用浸過75％酒精的脫脂棉擦凈丁基膠塞及疫苗瓶。
二、菌株恢復培養
1．吸取適量的菌種復溶液，滴人疫苗瓶內，輕輕振蕩，使凍于菌體溶解呈懸浮狀。
2．取適量菌體懸浮液(建議岢養菌種可適當加大用量)，移植于沙保羅瓊脂平板上，放置在30-35℃培養24-48小時.真菌在23-28℃培養箱中培養24-72小時（必要時可延長培養時間）
3．斜面（一代）放于4℃，并用封口膜密封平板，每半月轉接一次，代數一次類推，建議使用菌種代數不超過5代，5代后菌種應銷毀。
三、注意事項
1． 菌種活化前，請保存在2-8℃的環境下。
2． 嗜血桿菌、奈瑟氏菌屬等初代培養需要5-10%CO2環境和的培養基，建議使用培養基。
3． 厭氧菌的培養，如無特別說明，自開封至接種完成，均需以無氧氣體充填，以保持厭氧狀態。
4． 某些菌種經過冷凍干燥保存后，延遲期較長，需連續兩次繼代培養才能正常生長后使用。
ATCC 750菌種。熱帶念珠菌
Candida tropicalis (Caslani) Berkhout, anamorph (ATCC® 750™)
| Deposited As | Monilia tropicalis (Caslani) Caslani et Chalmers, anamorph |
|---|---|
| Strain Designations | [1909, ATCC 4563, ATCC 7349, CBS 94, CCRC 20520, DSY 59, IFO 1070, IFO 1400, JCM 1541, NRRL Y-12699, NRRL Y-12968, NRRL Y-607] |
| Application | Assay of chloro-8-quinolinol Bacterial resistance testing Degrades xylose D-xylose Fungus resistance testing adhesives Produces aspartic proteinases aspartyl proteinases Produces ethyl alcohol ethanol Produces xylitol Susceptibility disc testing Susceptibility testing Transformation host Produces xylitol from D-xylose Control strain for typing systems Produces ethanol from D-xylose Control strain to test oxygen availability in bioreactor in space laboratory Reference strain for Clinical and Laboratory Standards Institute(CLSI)-developed Antifungal Susceptibility Testing. |
| Biosafety Level | 1 ATCC 750菌種。熱帶念珠菌 |
| Product Format | freeze-dried |
| Storage Conditions | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C |
| Type Strain | yes (type strain) |
| Genotype | ade2/ade2 acp/acp delta::lambda/delta::lambda |
|---|---|
| Preceptrol® | yes |
| Genome Sequenced Strain | Yes |
| Comments | Fluconazole-sensitive Utilizes phenol, catechol and 3- and 4-methylcatechols as sole carbon source Esterase activity MIC of 2-hydroxychalcone, 75 mcg/ml Adherence to lysophospholipids Genome sequencing strain (Genolevures Consortium, France; Wellcome Trust Sanger Institute, UK). For additional infromation, see CLSI M27-A2 (Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standards-Second Edition). Mitochondrial genome sequenced strain |
| Medium | ATCC® Medium 200: YM agar or YM broth ATCC ® Medium 1245: YEPDATCC ® Medium 28: Emmons' modification of Sabouraud's agar |
|---|---|
| Growth Conditions | Temperature: 25°C Atmosphere: Typical aerobic |
