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中國倉鼠X小鼠B淋巴細胞雜交瘤;MR1 [derivative of HB-11048]
淋巴母細胞
半貼壁生長
動物用小鼠T細胞(Th1)進行免疫接種。中國倉鼠X小鼠B淋巴細胞雜交瘤;MR1 [derivative of HB-11048] 脾細胞與NS-1骨髓瘤細胞融合。 在活化的小鼠T細胞上檢測到表達的一個39KDa抗體蛋白。 1992年五月提交到ATCC的培養物污染了支原體。 其后代通過BM 細胞周期蛋白處理21天消除支原體。
RPMI 1640 (w/o Hepes) 胎牛血清,10%;0.05mM β-巰基乙醇
1:2。3天內可長滿。
不詳
*培養基+8%DMSO
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