產地：Roche
貨號：11667165001
Product Description
Form: clear, red, monophasic solution
Stability: stable for 24 months from the date of manufacture when stored unopened at +2 to +8oC in the original container
Contents
Ready-to-use solution
Application
RNA, DNA and protein from a single sample
TriPure Isolation Reagent can prepare total RNA, genomic DNA, and protein from a single biological sample. After purification:
The DNA-free total RNA is suitable for northern blotting, in vitro translation, RNase protection assay, cDNA synthesis, or RT-PCR.
The RNA-free DNA is suitable for PCR, restriction digests, Southern blotting, or cloning.
The denatured protein is suitable for SDS-PAGE and western blotting.
Benefits
Easy-to-use, because the red dye in the reagent simplifies identification of different phases.
Adapts easily to needs of different laboratories, because the reagent can process a wide variety of starting samples.
Simplifies isolation protocols for a variety of applications, because a single reagent can be used to isolate DNA-free RNA, RNA-free DNA, and proteins.
Increases yields of intact RNA, because the reagent creates an immediate chaotropic denaturing environment that eliminates endogenous RNase activity.